FCGR3A/CD16a human
SIGMA/SRP6436 - recombinant, expressed in HEK 293 cells, ≥98% (SDS-PAGE)
Synonym: FCG3; FCGR3; IGFR3
Product Type: Chemical
Immunoblotting FCGR3A/CD16a Cat. No. SRP6436, SDS PAGE of human recombinant FCGR3A/CD16a.
| assay | ≥98% (SDS-PAGE) |
| biological source | human |
| form | lyophilized |
| mol wt | calculated mol wt 22.7 kDa |
| observed mol wt 36-45 kDa by SDS-PAGE (DTT-reduced. Protein migrates due to glycosylation. Gly 17 is the predicted N-terminal.) | |
| packaging | pkg of 10 and 50 μg |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| storage temp. | −20°C |
| tag | 6-His tagged (C-terminus) |
| UniProt accession no. | P08637   |
| General description: | CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and Fcγ IIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16a antigen is also known as Low affinity immunoglobulin gamma Fc region receptor III-A, Fc-gamma RIII-α. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placentaltrophoblasts.CD1 |
| Physical form: | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |