COMP/TSP5 human
SIGMA/SRP6457 - recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
Synonym: EDM1; EPD1; MED; PSACH; THBS5
Product Type: Chemical
Immunoblotting COMP/TSP5 Cat. No. SRP6457, SDS PAGE of human recombinant COMP/TSP5.
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized |
| impurities | <1 EU/μg endotoxin (LAL test) |
| mol wt | calculated mol wt 82 kDa |
| observed mol wt 110-130 kDa (DTT-reduced. Protein migrates due to glycosylation. Gln 21 is the predicted N-terminal.) | |
| packaging | pkg of 10 and 50 μg |
| potency | 1 μg |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| storage temp. | −20°C |
| tag | 6-His tagged (C-terminus) |
| UniProt accession no. | P49747   |
| Biochem/physiol Actions: | Cartilage oligomeric matrix protein (COMP) plays a vital role in chondrocyte attachment, differentiation and cartilage extracellular matrix assembly. COMP levels vary in cartilage of osteoarthritis and rheumatoid arthritis patients compared with healthy individuals. Thus, COMP levels in serum and synovial fluid can be used as a biomarker for these diseases. Polymorphism in the gene leads to the development of two autosomal dominant skeletal dysplasias named, pseudoachondroplasia (PSACH) and multiple epiphyseal dysplasia type 1 (MED1). |
| General description: | Cartilage oligomeric matrix protein (COMP) also known as Thrombospondin-5 (TSP5), EDM1, EPD1, MED, PSACH, THBS5, which belongs to the thrombospondin family. COMP / TSP5 contain 4 EGF-like domains, 1 TSP C-terminal (TSPC) domain, 8 TSP type-3 repeats. Abundantly expressed in the chondrocyte extracellular matrix, and is also found in bone, tendon, ligament and synovium and blood vessels. COMP may play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. COMP can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Thrombospondin-5 could play a role in the pathogenesis of osteoarthritis. COMP is a marker of cartilage turnover. |
| General description: | Cartilage oligomeric matrix protein (COMP), also known as thrombospondin 5 (TSP5), is a key constituent of the extracellular matrix of the musculoskeletal system. It is encoded by the gene mapped to human chromosome 19p13.11. COMP/TSP5 is a fifth member of TSP family. It is a pentameric protein, characterized with an N-terminal coiled- coil oligomerization domain, four type II (EGF-like) domains, eight type III calmodulin like repeats (CLR) and a C-terminal globular domain (CTD). |
| Physical form: | Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |