ApoJ/Clusterin human
SIGMA/SRP6464 - recombinant, expressed in HEK 293 cells, ≥92% (SDS-PAGE)
Synonym: AAG4; Apolipoprotein J; CLI; CLU; KUB1; MGC24903; SGP-2; SGP2; SP-40; TRPM-2; TRPM2
Product Type: Chemical
Immunoblotting ApoJ/Clusterin Cat. No. SRP6464, SDS PAGE of human recombinant ApoJ/Clusterin.
| assay | ≥92% (SDS-PAGE) |
| biological source | human |
| form | lyophilized |
| impurities | <1 EU/μg endotoxin (LAL test) |
| mol wt | calculated mol wt 50.9 kDa (Asp23-Glu449, |
| observed mol wt 34-37 kDa by SDS-PAGE (DTT reduced. corresponding to the cleaved |
|
| packaging | pkg of 10 μg |
| pkg of 50 μg | |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| storage temp. | −20°C |
| tag | 6-His tagged (C-terminus) |
| UniProt accession no. | P10909   |
| General description: | Native Apolipoprotein J (ApoJ), also named Clusterin, is a heavily glycosylated, 75-80 kDa disulfide-linked heterodimeric protein. Despite being cloned since 1989, no genuine function has been attributed to ApoJ so far. The protein has been reportedly implicated in several diverse physiological processes such as sperm maturation, lipid transportation, complement inhibition, tissue remodeling, and membrane recycling, cell-cell and cell-substratum interactions, stabilization of stressed proteins in a folding-competent state and promotion or inhibition of apoptosis. ApoJ gene is differentially regulated by cytokines, growth factors and stress-inducing agents. Clusterin is up- or down regulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others. |
| Physical form: | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥92% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |