Transferrin human
SIGMA/SRP6472 - recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
Synonym: DKFZp781D0156; PRO1557; PRO2086; TF
Product Type: Chemical
Immunoblotting Transferrin Cat. No. SRP6472, SDS PAGE of human recombinant Transferrin.
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized |
| impurities | <1 EU/μg endotoxin (LAL test) |
| mol wt | calculated mol wt 76 kDa |
| observed mol wt 74 kDa by SDS-PAGE (DTT-reduced.) | |
| packaging | pkg of 100 μg |
| pkg of 20 μg | |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| storage temp. | −20°C |
| tag | 6-His tagged (C-terminus) |
| UniProt accession no. | P02787   |
| Application: | To supplement neurosphere culture medium and sphere differentiation medium. |
| Biochem/physiol Actions: | Transferrin is an iron-binding glycoprotein that primarily functions in the transport of plasma iron in vertebrates. It may also have a secondary function of facilitating cell proliferation. The binding of ferric iron to transferrin requires the presence of an anion, that functions as a bridge between the metal and the protein. |
| General description: | Transferrin is encoded by the TF gene that is mapped to human chromosome 3q22.1. It is a glycoprotein containing homologous iron-binding domains at N-terminal and C-terminal ends. Transferrin has a molecular weight of 80,000 Da and belongs to the transferrin superfamily, the members of which include lactoferrin, melanotransferrin, and ovotransferrin. |
| Physical form: | Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |