R spondin-1 human
SIGMA/SRP6487 - recombinant, expressed in HEK 293 cells, ≥98% (SDS-PAGE)
Synonym: CRISTIN-3; CRISTIN3; FLJ40906; R-spondin-1; RP11-566C13.1; RSPO; RSPO-1; RSPO1; Rspondin; Rspondin-1
Product Type: Chemical
Immunoblotting R spondin-1 Cat. No. SRP6487, SDS PAGE of human recombinant Rspondin-1.
| assay | ≥98% (SDS-PAGE) |
| biological source | human |
| form | lyophilized |
| impurities | <1 EU/μg endotoxin (LAL test) |
| mol wt | calculated mol wt 27.6 kDa |
| observed mol wt 40 kDa by SDS-PAGE (reducing) (Ser 21 is the predicted N-terminal .) | |
| packaging | pkg of 10 μg |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| storage temp. | −20°C |
| tag | 6-His tagged (C-terminus) |
| UniProt accession no. | Q2MKA7   |
| Application: | R spondin-1 human has been used to study the mechanism of action of methylphenidate (a drug to treat attention-deficit hyperactivity disorder), its effect on cell proliferation and maturation. |
| Biochem/physiol Actions: | RSPOs (roof plate-specific spondin) plays a crucial role in embryogenesis and tissue homeostasis. During the developmental stages of ovary, RSPO1 becomes a necessary factor for sex determination and follicular cell progenitor differentiation. The RSPO1 gene expression is also predicted to initiate ovarian cancer. It has a supportive role in cell proliferation, differentiation, and survival. RSPO1 is centrally associated with intestinal stem cell induction. |
| General description: | R-spondin-1, also known as Roof plate-specific Spondin 1 (RSPO1) and cysteine rich and single thrombospondin domain containing protein 3 (Cristin 3), is a secreted protein which belongs to the R-Spondin family and encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. All R spondins regulate Wnt/β-catenin signaling, but have distinct expression patterns. Like other R-Spondins, R-Spondin-1 contains two adjacent cysteine rich furin like domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furin like domains are needed for β-catenin stabilization. A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus. Potential isoforms of 200 and 236 aa have an alternate, shorter N-terminus or are missing aa 146-208, respectively. |
| Physical form: | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |