KITLG/SCF from mouse
SIGMA/SRP6488 - recombinant, expressed in HEK 293 cells, ≥98% (SDS-PAGE)
Synonym: FPH2; KL; KL-1; Kitl; MGF; SF; SHEP7
Product Type: Chemical
assay | ≥98% (SDS-PAGE) |
biological source | mouse |
form | lyophilized |
impurities | <1 EU/μg endotoxin (LAL test) |
mol wt | calculated mol wt 19.1 kDa |
observed mol wt 19-30 kDa (DTT-reduced. Lys 26 is the predicted N-terminal.) | |
packaging | pkg of 100 μg |
recombinant | expressed in HEK 293 cells |
shipped in | wet ice |
storage temp. | −20°C |
tag | 6-His tagged (C-terminus) |
UniProt accession no. | P20826 ![]() ![]() |
General description: | Kit ligand (KITLG) also known as stem cell factor (SCF), mast cell growth factor (MGF), steel factor (SF), which belongs to the SCF family, and is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. KITLG is the ligand for the receptor-type protein-tyrosine kinase KIT. SCF / MGF plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG / SCF binding can activate several signaling pathways. KITLG / SF Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG / SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG / SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG / SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1, 4, 5 - triphosphate. KITLG / SCF acts synergistically with other cytokines, probably interleukins. |
Physical form: | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization. |
Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥98% (SDS-PAGE) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |