B7-2/CD86 human
SIGMA/SRP6501 - recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
Synonym: B70; CD28LG2; LAB72; MGC34413
Product Type: Chemical
assay | ≥95% (SDS-PAGE) |
biological source | human |
form | lyophilized |
impurities | <1 EU/μg endotoxin (LAL test) |
mol wt | calculated mol wt 26.7 kDa |
observed mol wt 43-60 kDa (DTT-reduced.Protein migrates due to glycosylation. Gly 24 is the predicted N-terminal.) | |
packaging | pkg of 10 μg |
pkg of 50 μg | |
recombinant | expressed in HEK 293 cells |
shipped in | wet ice |
storage temp. | −20°C |
tag | 6-His tagged (C-terminus) |
UniProt accession no. | P42081 |
General description: | Cluster of Differentiation 86 (CD86) is also known as B-lymphocyte activation antigen B7-2, is a type I membrane protein that is a member of the immunoglobulin superfamily, and is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B72 is expressed at low levels on monocytes and can be upregulated through interferon γ. CD86 is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD86 works in tandem with CD80 to prime T cells. Recent study has revealed that B7-2 promotes the generation of a mature APC repertoire and promotes APC function and survival. Furthermore, the B7 proteins are also involved in innate immune responses by activating NF-κB-signaling pathway in macrophages. CD86 thus is regarded as a promising candidate for immune therapy. CD86+ macrophages in Hodgkin lymphoma patients are an independent marker for potential nonresponse to firstline-therapy. |
Physical form: | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization. |
Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥95% (SDS-PAGE) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |