G6PD human
SIGMA/SRP6505 - recombinant, expressed in E. coli, ≥95% (SDS-PAGE)
Synonym: Glucose-6-phosphate 1-dehydrogenase; Zwf
Product Type: Chemical
Immunoblotting G6PD Cat. No. SRP6505, SDS PAGE of human recombinant G6PD.
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| concentration | 0.5 mg/mL |
| form | liquid |
| mol wt | 61.4 kDa (515 aa, 1- 515 aa) |
| packaging | pkg of 100 μg |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| UniProt accession no. | P11413   |
| Analysis Note: | The biological activity is > 7 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 μmole D-glucose-6-phosphate to 6-phospho-Dgluconate per min in the presence of beta-NADP at pH 7.4 at 25 °C. |
| Biochem/physiol Actions: | G6PD (glucose-6-phosphate dehydrogenase) enzyme catalyzes the oxidation of glucose-6-phosphate to 6-phosphogluconolactone, reducing NADP to NADPH. It therefore, catalyzes the first step of hexose monophosphate pathway (HMP). As it is responsible for the oxidation of glucose-6-phosphate, and thus, confers protection against oxidative damage in erythrocytes. Its deficiency is highly heterogenous, with around 190 variants being reported. The deficiency of G6PD was discovered while studying hemolytic anemia. G6PD also results in hereditary nonspherocytic hemolytic anemia. |
| General description: | G6PD (glucose-6-phosphate dehydrogenase) gene is mapped to human chromosome Xq28, and spans 16.2kb. One G6PD monomer is composed of 515 amino acids with a predicted molecular weight of 59,256Da. The active enzyme exists as a dimer, and contains an NADP molecule tightly bound to it. |
| Physical form: | 0.5 mg/mL solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1 mM PMSF, 2 mM EDTA, 2 mM DTT, 200 mM NaCl. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 |
| Precautionary statements | P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |