Lactoferrin from human milk
SIGMA/SRP6519 - ≥95% (SDS-PAGE)
Synonym: Lactotransferrin; Talalactoferrin
Product Type: Chemical
Immunoblotting Lactoferrin, human Milk Cat. No. SRP6519, 4-12% Bis-Tris NuPAGE gel of Lactoferrin, human milk. Lanes 1. Lactoferrin - 5 μg (reduced/heated) 2. Lactoferrin - 10 μg (reduced/heated) 3. Lactoferrin - 20 μg (reduced/heated) 4. LMW Standard 5. Lactoferrin - 5 μg (non-reduced/no heat) 6. Lactoferrin - 10 μg (non-reduced/no heat) 7. Lactoferrin - 20 μg (non-reduced/no heat)
| assay | ≥95% (SDS-PAGE) |
| biological source | human milk |
| form | frozen liquid |
| mol wt | 82.4 kDa |
| packaging | pkg of 10 mg |
| shipped in | dry ice |
| storage temp. | −70°C |
| technique(s) | ELISA: suitable |
| UniProt accession no. | 4057   |
| Application: | Lactoferrin from human milk has been used as a standard in standard curve generation for quantifying recombinant lactoferrin in enzyme-linked immunosorbent assay (ELISA). It has also been used as a positive control in sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE ). |
| Biochem/physiol Actions: | Lactoferrin is known to regulate intestinal iron absorption, and to participate in the defense against bacteria. It functions in the innate immune system via the activation of nuclear factor-κB (NF-κB). This activation occurs via Toll-like receptor 4 pathway due to the interference of lactoferrin with the lipopolysaccharide-stimul |
| General description: | Lactoferrin gene is mapped to human chromosome 3p21.31. |
| General description: | Lactoferrin is a glycoprotein present in exocrine secretions and in the secondary granules of polymorphonuclear neutrophils. It is the iron binding protein in milk. Human lactoferrin belongs to the family of transferrins. It is found to be expressed in several glandular epithelial cells and skin epidermal keratinocytes. |
| Physical form: | Frozen in 50 mM Tris and 0.2 M NaCl, pH 8.0 . |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |