Ubiquitin-Fluorescein-labeled (FLR-Ub)
SIGMA/SRP6521 - ≥95% (SDS-PAGE)
Synonym: CEP80; HUBCEP80; RPS27A; Ribosomal Protein S27a; UBA80; UBB; UBCEP1; UBCEP80
Product Type: Chemical
| assay | ≥95% (SDS-PAGE) |
| concentration | 8 mg/mL |
| form | liquid |
| mol wt | 9.009 kDa |
| packaging | pkg of 50 μg |
| shipped in | dry ice |
| storage temp. | −70°C |
| Biochem/physiol Actions: | Ribosomal protein S27a (RPS27a) plays a major role in ribosome biogenesis and post-translational modifications of proteins. It also stimulates proliferation, modulates cell cycle and prevents apoptosis of leukemia cells. |
| General description: | Post-translational modification of proteins by ubiquitin (Ub) is a key regulatory process that impacts almost all cellular functions. Apart from the established role of Ub in protein degradation, it is implicated in cell signaling, DNA damage response, protein trafficking, cell-cycle progression, inflammation, immune response and regulation of apoptosis. Ubiquitylation occurs through isopeptide linkage between the C-terminus of Ub and the e-amino group of a lysine (Lys) residue on the target substrate. Ub itself has seven Lys residues (6, 11, 27, 29, 33, 48, and 63), any of which can participate in further ubiquitylation, generating polyUb chains. Monitoring the ubiquitylation of target proteins or the growth of polyubiquitin chains has traditionally been carried out with either radiolabeled or epitope-tagged ubiquitin requiring long and laborious detection methods. Fluorescently labeled ubiquitin provides a rapid, facile technique for studying ubiquitin conjugation in vitro. Unlike others, Thefluorescein-labeled ubiquitin carries a single fluorescein molecule attached at a defined location and avoids modification of either the N-terminus or Lys side chains. |
| General description: | The ribosomal protein S27a (RPS27a) gene codes for a fusion protein, which consists of ubiquitin (Ub) at the N terminus and ribosomal protein (RP) S27a at the C terminus. It is located on human chromosome 2p16. |
| Physical form: | ≥ 8 mg/mL in PBS. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |