Ubiquitin-TAMRA-labeled (TMR-Ub)
SIGMA/SRP6522 - ≥95% (SDS-PAGE)
Synonym: CEP80; HUBCEP80; RPS27A; Ribosomal Protein S27a; UBA80; UBB; UBCEP1; UBCEP80
Product Type: Chemical
assay | ≥95% (SDS-PAGE) |
concentration | 8 mg/mL |
form | liquid |
mol wt | 9.133 kDa |
packaging | pkg of 50 μg |
shipped in | dry ice |
storage temp. | −70°C |
General description: | Post-translational modification of proteins by ubiquitin (Ub) is a key regulatory process that impacts almost all cellular functions. Ubiquitylation occurs through isopeptide linkage between the C-terminus of Ub and the e-amino group of a lysine (Lys) residue on the target substrate [1]. Ub itself has seven Lys residues (6, 11, 27, 29, 33, 48, and 63), any of which can participate in further ubiquitylation, generating polyUb chains [2, 3]. Monitoring the ubiquitylation of target proteins or the growth of polyubiquitin chains has traditionally been carried out with either radiolabeled or epitope-tagged ubiquitin requiring long and laborious detection methods. Fluorescently labeled ubiquitin provides a rapid, facile technique for studying ubiquitin conjugation in vitro. Unlike others, TheTAMRA-labeled ubiquitin carries a single TAMRA molecule attached at a defined location and avoids modification of either the N-terminus or Lys side chains. |
Physical form: | ≥ 8 mg/mL in PBS. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥95% (SDS-PAGE) |
Storage Temp. | −70°C |
UNSPSC | 12352200 |