NQO1 human
SIGMA/SRP6539 - recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Synonym: Azoreductase; DT-Diaphorase (DTD); Dioxin-inducible 1; Disphorase-4 (DIA4); Menadione Reductase; NAD(P)H Dehydrogenase (Quinine) 1 (NQO1); NAD(P)H Quinone Oxireductase; Phylloquinone Reductase; Quinone Oxidoreductase 1; Quinone Reductase 1 (QR1)
Product Type: Chemical
| assay | ≥90% (SDS-PAGE) |
| biological source | human |
| form | liquid |
| impurities | <0.1 EU/μg endotoxin (LAL test) |
| mol wt | ~30 kDa (Human NQO1 aa 1-274 is fused at the C-terminus to a His-tag.) |
| packaging | pkg of 10 and 50 μg |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| UniProt accession no. | P15559   |
| Biochem/physiol Actions: | NQO1 (NAD(P)H quinone dehydrogenase 1) uses NADH or NADPH as a reducing cofactor, and is characterized by its inhibition by dicoumarol. It is thought to confer protection against natural and exogenous quinones. It is involved in the conversion of benzene derived quinones to less toxic hydroquinones, and participates in benzene-associated hematotoxicity. It is involved in the reduction and eventual activation of few chemotherapeutic drugs and of environmental carcinogens, such as heterocyclic amines, nitroaromatic compounds, and possibly cigarette smoke particulate. C609T null variant of this gene is linked with breast cancer susceptibility in north Indian patients. NQO1 is up-regulated in serous ovarian carcinoma, and this expression is linked with poor prognosis; hence, this gene has potential as biomarker for the same. |
| General description: | NQO1 (NAD(P)H quinone dehydrogenase 1) is an obligate two-electron reductase. It is a cytoplasmic, homodimeric protein, with one FAD molecule per monomer. This gene is localized to human chromosome 16q23. It shows a wide range of tissue expression. |
| Physical form: | 0.2 μm-filtered solution in 50 mM Tris-Cl, pH 8.0, containing 1 mM DTT. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H319 |
| Precautionary statements | P264 - P280 - P305 + P351 + P338 - P337 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |