Procathepsin L human
SIGMA/SRP6546 - recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Synonym: CTSL; CTSL1
Product Type: Chemical
Immunoblotting Procathepsin L Cat. No. SRP6546, SDS-PAGE (12%) of h-Procathepsin L. Lanes 1. Protein Marker 2. Human Procathepsin L (10 μg) 3. Human Procathepsin L (15 μg) 4. Human Procathepsin L (20 μg)
Immunoblotting Procathepsin L Cat. No. SRP6546, SDS-PAGE (12%) of activation of h-Procathepsin L. Lanes 1. Protein Marker 2. Human Procathepsin L (5 μg) 3 to 5. Human Procathepsin L (5 μg) after 1, 15 and 30 min of activation at low Ph.
| assay | ≥90% (SDS-PAGE) |
| biological source | human |
| concentration | 1 mg/mL |
| form | liquid |
| mol wt | 39.4 kDa (18-333 aa + N-terminal poly-his tag.) |
| packaging | pkg of 1 mg |
| pkg of 100 μg | |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −70°C |
| UniProt accession no. | P07711   |
| General description: | Cathepsin L is a lysosomal protease that can be secreted from the cell and is implicated to play a role in protein degradation, arthritis, apoptosis, and cancer. TheProcathepsin L can be auto-catalytically activated to mature Cathepsin L (~24 kDa) by adding an equal volume of 0.2 M NaOAc, 5 mM EDTA pH 4.0 containing fresh 5 mM DTT at RT. The enzyme is fully activated within 30 min of incubation at RT. After activation mature Cathepsin L is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, methylmethanthiosulfonate (1mM final concentration; MeS-SO2Me; MMTS) could be added and sample should be frozen at -80°C. The activity can be then restored by adding L-cysteine (3 M excess over MMTS) to the enzyme solution. |
| Physical form: | 1 mg/mL in 25 mM Na2HPO4, 500 mM NaCl, pH 7.0. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |