Thrombin Active (High Activity) from bovine plasma
SIGMA/SRP6556 - ≥98% (SDS-PAGE)
Synonym: Activated Factor IIa
Product Type: Chemical
| assay | ≥98% (SDS-PAGE) |
| biological source | bovine plasma |
| form | lyophilized |
| mol wt | 37 kDa |
| packaging | pkg of 10,000 units |
| pkg of 100,000 units | |
| pkg of 1000 units | |
| potency | >1500 units/mg |
| shipped in | wet ice |
| storage temp. | −20°C |
| UniProt accession no. | P00735   |
| Biochem/physiol Actions: | Thrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity. Thrombin enzyme (Activated Factor IIa) also participates in inflammation and has mitogenic role on vascular cells. It is also involved in the activation of protein C which is needed for the inactivation of many procoagulant enzymes. |
| General description: | Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. |
| Physical form: | Sterile filtered and lyophilized with Mannitol and Sodium Chloride. |
| Reconstitution: | Reconstitute in sterile water (100 U/mL) with 0.9% NaCl. It forms a clear solution. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |