Sushi Peptide S3

SIGMA/SRP6562 - recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonym: Factor C Sushi 3; Sushi 3

Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-SRP6562-20UG	20 µg	$0.00 1/EA CALL FOR PRICE	Add To Favorites
45-SRP6562-100UG	100 µg	$0.00 1/EA CALL FOR PRICE	Add To Favorites
45-SRP6562-1MG	1 mg	$0.00 1/EA CALL FOR PRICE	Add To Favorites

Immunoblotting Sushi Peptide S3, bacterial Cat. No. SRP6562, SDS-PAGE (15%) of purified Sushi Peptide S3. Lanes 1. Protein Marker 2. 20 μg Sushi Peptide S3 3. 30 μg Sushi Peptide S3 4. 40 μg Sushi Peptide S3

Properties
Descriptions
Safety Info.
Basic Data

assay	≥90% (SDS-PAGE)
form	lyophilized powder
mol wt	16.1 kDa
packaging	pkg of 1 mg
	pkg of 100 μg
	pkg of 20 μg
recombinant	expressed in E. coli
shipped in	wet ice
storage temp.	−20°C

General description:	TheSushi Peptide S3 is a trimer of one of the high endotoxin-binding domains, Sushi 3 (or S3) within Factor C, a lipopolysaccharide (LPS)-sensitive serine protease of the horseshoe crab (Limulus Polyphemus). S3 display detergent-like properties in disrupting LPS aggregates, with specificity for palmitoyl-oleoyl-phosphatidylglycerol (POPG) resulting from electrostatic and hydrophobic forces between the peptides and the bacterial lipids. During interaction with POPG, the S3 resumes a mixture of α-helix and β-sheet structures. The unsaturated nature of POPG confers fluidity and enhances insertion of the peptides into the lipid bilayer, causing maximal disruption of the bacterial membrane. In short, peptide S3 can bind to lipopolysaccharide (LPS) and inhibit the growth of Gram-negative bacteria without affecting mammalian cells. It has been shown that endotoxin activates Factor C-based catalytic coagulation cascade resulting in the gelation of Limulus blood. This process is the basis of Limulus Amebocyte Lysate (LAL) endotoxin detection method.
Physical form:	Lyophilized from 50 mM Tris. HCl and 100 mM NaCl (pH 8.0).
Reconstitution:	Reconstitute with water. Recommended conc: 0.1 - 1 mg/mL.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥90% (SDS-PAGE)
Storage Temp.	−20°C
UNSPSC	12352200

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