BrdT (257-382) human
SIGMA/SRP6563 - recombinant, expressed in E. coli, ≥98% (SDS-PAGE)
Synonym: CT9; Cancer/testis antigen 9; RING3-like protein
Product Type: Chemical
Immunoblotting BrdTdomain (257-382 aa) Cat. No. SRP6563, SDS-PAGE (15%) of purified BrdT protein. Lanes 1. Marker. 2. BrdT protein (257-382 aa) - 10 μg 3. BrdT protein (257-382 aa) - 25 μg 4. BrdT protein (257-382 aa) - 50 μg
| assay | ≥98% (SDS-PAGE) |
| biological source | human |
| form | liquid |
| mol wt | 14.9 kDa (126 aa, 257-382 aa + NT His Tag) |
| NCBI accession no. | NM_207189   |
| packaging | pkg of 1 mg |
| pkg of 100 μg | |
| pkg of 20 μg | |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | Q58F21 |
| General description: | BrdT is similar to the RING3 protein family. It possesses 2 bromodomain motifs and a PEST sequence. The bromodomain is found in proteins that regulate transcription. BrdT drives a meiotic and post-meiotic gene expression program. It also controls the genome-wide post-meiotic genome reorganization that occurs after histone hyperacetylation in elongating spermatids. It may play a role in the transcriptional regulation of spermatogenesis. It also seems to have a structural ATP-independent role in the reorganization of acetylated chromatin. Therecombinant protein includes Bromo 1 domain (257-382 aa) with N-terminal His-tag. |
| Physical form: | rh-BrdT is supplied as a solution in 50 mM Tris, pH 8.0, 150 mM NaCl and 10% Glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |