BRPF3 (576-701) GST tag human
SIGMA/SRP6580 - recombinant, expressed in E. coli, ≥95% (SDS-PAGE)
Synonym: Bromodomain and PHD Finger Containing 3; KIAA1286; MGC:58603
Product Type: Chemical
Immunoblotting BRPF3 (576-701 aa) (GST-tagged) Cat. No. SRP6580, SDS PAGE of human recombinant BRPF3 bromodomain. Lanes 1. Marker 2. BRPF3 (8 μg) 3. BRPF3 (4 μg) 4. BRPF3 (2 μg)
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | liquid |
| mol wt | 41.2 kDa (576-701 aa + NT GST Tag) |
| packaging | pkg of 100 μg |
| pkg of 20 μg | |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | Q9ULD4   |
| General description: | The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. Bromodomain and PHD finger containing 3 (BRPF3) is a component of the MOZ/MORF histone acetyltransferase (HAT) complex. The addition of BRPF proteins to MOZ/MORF increases its HAT activity.3 Consequently, BRPF3 is likely to play a role in regulation of transcriptional activation by MOZ/MORF. This product contains the first bromodomain of BRPF3. |
| Physical form: | 50 mM Tris, pH 7.5, containing 500 mM sodium chloride, 5 mM β-mercaptoethanol and 5% glycerol. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H319 - H412 |
| Precautionary statements | P273 - P280 - P305 + P351 + P338 - P333 + P313 - P337 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |