EED (1-441) human
SIGMA/SRP6583 - recombinant, expressed in E. coli, ≥95% (SDS-PAGE)
Synonym: Embryonic Ectoderm Development
Product Type: Chemical
Immunoblotting EED (1- 441aa) Cat. No. SRP6583, SDS PAGE of human recombinant EED. Lanes 1. Marker 2. Pure EED (0.5 μg) 3. Pure EED (1 μg) 4. Pure EED (2 μg) 5. Pure EED (5 μg) 6. Pure EED (10 μg)
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | liquid |
| mol wt | 53.5 kDa (1- 441 aa + NT His Tag) |
| packaging | pkg of 25 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | O75530   |
| General description: | Polycomb protein Embryonic Ectoderm Development (EED) is a WD repeat containing member of the Polycomb-group (PcG) family. The EED protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. EED is an established transcriptional repressor, as a novel NIPP1 interactor. NIPP1 only interacted with full-length EED, whereas two EED interaction domains were mapped to the central and COOH-terminal thirds of NIPP1. In mammalian cells, EED is present as four distinct isoforms, which are believed to be produced by utilizing four distinct, in-frame translation start sites in a common EED mRNA. The extra sex combs (esc) gene of Drosophila and its mammalian homologue embryonic ectoderm development (EED) play pivotal roles in establishing Polycomb-group (Pc-G) mediated transcriptional silencing of regulatory genes during early development. EED/Sox2 regulatory loop contributes to the maintenance of self-renewal in embryonic stem (ES) cells by controlling histone methylation and acetylation. EED has been defined by the inability of embryos homozygous for certain c deletions to develop beyond the early stages of gastrulation. |
| Physical form: | 50 mM sodium phosphate, pH 7.2, containing 100 mM sodium chloride and 20% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |