Histone H2B (1-123) from Xanopus sp.
SIGMA/SRP6585 - recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Synonym: HIST1H2BA; Histone H2B type 1-A; TSH2B
Product Type: Chemical
Immunoblotting Histone H2B (1-123 aa), Xenopus Cat. No. SRP6585, SDS PAGE of Xenopus recombinant H2B. Lanes 1. Marker 2. Purified H2B (1 μg) 3. Purified H2B (2 μg)
| assay | ≥90% (SDS-PAGE) |
| biological source | Xenopus laevis |
| form | lyophilized powder |
| mol wt | 13.7 kDa (1-123 aa) |
| packaging | pkg of 100 and 250 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | Q96A08   |
| General description: | H2A is a core component of nucleosome. A nucleosome is the basic repeating unit of chromatin in which 146 base pairs of DNA wrap twice around an octamer of histones. The octamer is composed of two of each histone H2A, H2B, H3, and H4. DNA accessibility is regulated via a complex set of post-translational modifications of these histones, also called histone code, and nucleosome remodeling. Histones H2A and H2B form a dimer. Histones H3 and H4 form a tetramer. The combination of two H2A/H2B dimers and one H3/H4 tetramer create the nucleosome core. Histone H2B undergoes many modifications which include acetylation, phosphorylation, ubiquitylation, sumoylation, and biotinylation that are important for regulation of gene transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. |
| Physical form: | Lyophilized powder. Recommended buffer is 50 mM sodium phosphate, pH 7.2, containing 300 mM sodium chloride, 1 mM DTT, 1 mM EDTA and 20% glycerol. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H319 |
| Precautionary statements | P264 - P280 - P305 + P351 + P338 - P337 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |