JMJD2 (888-1023) GST tag human
SIGMA/SRP6591 - recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Synonym: JHDM3A; Jumonji Domain Containing 2A; KDM4A
Product Type: Chemical
Immunoblotting JMJD2A (888-1023 aa) (GST-tagged) Cat. No. SRP6591, SDS PAGE of human recombinant JMJD2A Tudor Domains. Lanes 1. Marker 2. JMJD2A Tudor (5 μg) 3. JMJD2A Tudor (2.5 μg)
| assay | ≥90% (SDS-PAGE) |
| biological source | human |
| form | liquid |
| mol wt | 42.2 kDa (888-1023 aa, NT GST Tag) |
| packaging | pkg of 20 and 50 μg |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | O75164   |
| General description: | Tudor domains are small protein structural motifs of about ~50 amino acids related to the “royal family” of methyl readers, which also includes chromo, MBT, PWWP, and Agenet-like domains. Tudor domains occur either alone, in tandem, or with other domains and are found in many proteins that are involved in RNA metabolism, germ cell development, transposon silencing, DNA damage response, histone modification, and chromatin remodeling. The tudor domains recognize symmetric methylated arginine or methylated lysine residues. JMJD2A catalyzes the demethylation of trimethylated histone H3 at lysine residues 9 and 36 (H3K9me3 and H3K36me3). However, the tudor domain of this protein has been shown to bind histone H3K4me, H3K9me3, and H3K20me2/3. Like other JmjC protein hydroxylase family members, JMJD2A is an α-ketoglutarate-dependent Fe (II) oxygenase. This product contains the tandem tudor domains of JMJD2A. |
| Physical form: | 50 mM Tris-HCl, pH 8.0, 150 mM sodium chloride, and 20% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |