MMP-3 human
SIGMA/SRP7783 - recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)
Synonym: EC 3; Matrix metalloproteinase-3; Stromelysin-1
CAS Number: 79955-99-0
MDL Number: MFCD00282364
Product Type: Chemical
| assay | ≥95% (HPLC) |
| ≥95% (SDS-PAGE) | |
| biological source | human |
| form | lyophilized |
| impurities | endotoxin, tested |
| mol wt | ~19.5 kDa |
| NCBI accession no. | P08254   |
| packaging | pkg of 10 μg |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | western blot (chemiluminescent): suitable |
| Application: | MCP-1 has been used to induce chemotaxis in mice macrophages. |
| Application: | MMP-3 human has been used to develop a simple and sensitive electrogenerated chemiluminescence (electrochemiluminescence (ECL)) peptide-based bioassay for the detection of matrix metalloproteinases releasing from living cells. |
| Biochem/physiol Actions: | Matrix metallopeptidase 3 (MMP3) has a broad substrate specificity and is capable of degrading fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. It participates in wound repair, progression of atherosclerosis, tissue remodeling and tumor initiation. MMP3 variants can be associated with hypertrophy of interventricular septum or hypertrophic cardiomyopathy. |
| Biochem/physiol Actions: | The gene JE, also referred to as MCP-1 (monocyte chemoattractant protein-1), encodes a secreted chemokine that functions as a chemoattractant for monocytes and may activate their anti-tumor properties. This protein may serve as a potential therapeutic anticancer agent. |
| General description: | Active MMP-3 catalytic domain from human cDNA, expressed in E. coli and purified using proprietary technologies. The active MMP-3 is very useful in studying enzyme kinetics, cleave target substrates, and screen for inhibitors. |
| General description: | The gene JE is an early growth response gene that was first identified in mouse 3T3 cells. The gene is mapped to mouse chromosome 11. It encodes a member of the CC subfamily, the members of which contain characteristic two adjacent cysteine residues. |
| Physical form: | Lyophilized powder. |
| Reconstitution: | Centrifuge the vial prior to opening. Avoid freeze-thaw cycles. |
| Reconstitution: | Reconstitute in sterile PBS to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (HPLC); ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |