Calreticulin human
SIGMA/SRP8001 - recombinant, expressed in E. coli, His tagged, >90% (SDS-PAGE)
Synonym: CRP55; Calregulin; ERp60; Endoplasmic reticulum resident protein 60; grp60
Product Type: Chemical
assay | >90% (SDS-PAGE) |
biological source | human |
form | liquid |
impurities | <1 EU/μg endotoxin, tested |
mol wt | ~55 kDa by SDS-PAGE |
packaging | pkg of 10 μg |
recombinant | expressed in E. coli |
shipped in | wet ice |
storage condition | avoid repeated freeze/thaw cycles |
storage temp. | −20°C |
tag | His tagged |
technique(s) | calcium flux assay: suitable |
UniProt accession no. | P27797 |
Application: | Human calreticulin has been used in a study to determine the effects of uncoupling protein 4 (UCP4) on the expression of proteins in the store-operated Ca2+ entry pathway. |
Biochem/physiol Actions: | Calreticulin is involved in regulation of intracellular Ca2+ homoeostasis and ER Ca2+ capacity. It constitutes together with calnexin and ERp57 the ′calreticulin/calnexin cycle′ that is responsible for folding and quality control of newly synthesized glycoproteins. Calreticulin has been implicated to play a role in many biological systems, including functions inside and outside the ER, indicating that the protein is a multi-process molecule. Recently, Calreticulin was shown to enhance the merger of macrophages and tumor cells, increasing phagocytosis. |
General description: | Calreticulin (CALR) is expressed in the endoplasmic reticulum. The gene encoding it is localized on human chromosome 19p13.13. |
Other Notes: | Human calreticulin (aa18-417) is fused at the C-terminus to a His-tag. |
Physical form: | Solution in 55 mM TRIS-HCl, pH 8.2, containing 150 mM NaCl. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | >90% (SDS-PAGE) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |