Progranulin mouse
SIGMA/SRP8015 - recombinant, expressed in HEK 293 cells, untagged, >95% (SDS-PAGE)
Synonym: GPx-1; PEPI; Proepithelin
Product Type: Chemical
Immunoblotting Progranulin Mouse: Cat. No. SRP8015: The effects on phospho-ERK1/2 and non-phospho-ERK1/2 by Progranulin Mouse in neuronal differentiated Mouse P19 cells. Undifferentiated Mouse P19 embryonal carcinoma cells were induced to differentiated in 1 βM retinoic acid (RA) in α-minimum essential medium (αMEM) containing 10% heat-treated fetal bovine serum on bacterial grade plates for 3-4 days to allow aggregates to form (generation of embryonic bodies). The aggregates were then plated out tissue culture grade plates in the absence of RA for 3-4 days. To examine the induction of signal of phospho-p44/42 MAPK and p44/42 MAP kinase, reactions were carried out at 37 °C over 0, 5, 10, 30, 60, 120 min, respectively by adding the recombinant protein (500 ng/mL) to the neuronal differentiated Mouse P19 embryonal carcinoma cells, which were maintained with serum starvation for 24 hours. Treatment with Progranulin Mouse was performed in lanes 1, 2, 3, 4, 5, and 6 over 0, 5, 10, 30, 60, 120 min, respectively.
| assay | >95% (SDS-PAGE) |
| biological source | mouse |
| color | white |
| conjugate | untagged |
| form | lyophilized |
| impurities | <0.1 EU/μg endotoxin, tested |
| mol wt | ~65 kDa by SDS-PAGE |
| packaging | pkg of 10 μg |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| storage temp. | −20°C |
| UniProt accession no. | P28798   |
| General description: | Progranulin (PGRN) is a widely expressed pluripotent growth factor which plays a role in processes such as development, wound repair and inflammation by activating signaling cascades that control cell cycle progression and cell motility. Its function in the central nervous system is of interest, as mutations in the PGRN gene were found in cases of frontotemporal degeneration (FTLD). In addition, PGRN has also been linked to tumorigenesis. Progranulin is a biomarker for FTLD, other types of Alzheimer′s Disease (AD) and potentially for MCI (Mild Cognitive Impairment). Additionally, PGRN is described as a new ligand of TNF receptors and a potential therapeutic against inflammatory disease like arthritis. |
| Other Notes: | Signal peptide and mouse progranulin (aa 1-589) is untagged. Reflects the native sequence with no additional aa. |
| Physical form: | Lyophilized from 0.2 μm-filtered solution in PBS, pH 7.2. |
| Reconstitution: | Reconstitute with 100 μL deionized water. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |