Progranulin mouse
SIGMA/SRP8016 - recombinant, expressed in HEK 293 cells, FLAG® tagged, >95% (SDS-PAGE)
Synonym: PEPI; Proepithelin
Product Type: Chemical
Immunoblotting Progranulin Mouse: Cat. No. SRP8016: ERK phosphorylation induced by Progranulin Mouse-FLAG® in MCF10A cells. To examine the signal of phospho-p44/42 MAPK and p44/42 MAP kinase, reactions were carried out at 37 °C over 0, 5, 10, 30 min, respectively, by adding Progranulin Mouse (500 ng/mL) to the MCF10A Human breast epithelial cells, which were maintained with serum starvation for 24 hours. Proteins in lanes 1, 2, 3, 4 were subjected to MCF10A Human breast epithelial cell treatments over 0, 5, 10, 30 min, respectively.
| assay | >95% (SDS-PAGE) |
| biological source | mouse |
| color | clear |
| concentration | ≥0.2 mg/mL |
| form | liquid |
| impurities | <0.1 EU/μg endotoxin, tested |
| manufacturer/tradename | Sigma-Aldrich |
| mol wt | ~70 kDa by SDS-PAGE |
| packaging | pkg of 10 μg |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| tag | FLAG® tagged |
| UniProt accession no. | P28798   |
| General description: | Progranulin (PGRN) is a widely expressed pluripotent growth factor which plays a role in processes such as development, wound repair and inflammation by activating signaling cascades that control cell cycle progression and cell motility. Its function in the central nervous system is of interest, as mutations in the PGRN gene were found in cases of frontotemporal degeneration (FTLD). In addition, PGRN has also been linked to tumorigenesis. Progranulin is a biomarker for FTLD, other types of Alzheimer′s Disease (AD) and potentially for MCI (Mild Cognitive Impairment). Additionally, PGRN is described as a new ligand of TNF receptors and a potential therapeutic against inflammatory disease like arthritis. |
| Legal Information: | FLAG is a registered trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | Signal peptide and mouse progranulin (aa 1-589) are fused at the C-terminus to a FLAG®-tag. |
| Physical form: | Solution in PBS. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |