Flagellin from Salmonella typhimurium
SIGMA/SRP8029 - >95% (SDS-PAGE)
Synonym: FliC; Intelectin-1
CAS Number: 12777-81-0
Product Type: Chemical
Histogram Flagellin from Salmonella typhimurium protein: Cat. No. SRP8029: Flagellin from Salmonella typhimurium activates TLR5 In vivo. Method: Mice WT are injected intravenously with indicated doses of Flagellin. After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected intravenously with 10 βg of Flagellin, but no expression of CCL20 and IL-6 could be observed.
| assay | >95% (SDS-PAGE) |
| biological source | Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |
| color | white |
| form | lyophilized |
| impurities | <0.01 EU/μg endotoxin, tested |
| mol wt | ~50 kDa by SDS-PAGE |
| packaging | pkg of 10 μg |
| shipped in | wet ice |
| storage temp. | −20°C |
| UniProt accession no. | P06179   |
| Biochem/physiol Actions: | FliC (flagellin) can activate the innate immune system by TLR5 (toll like receptor 5) and is an important proinflammatory molecule. FliC also induces the production of antibodies and is identified as an antigen by CD4+ T cells. |
| Biochem/physiol Actions: | It also stimulates the production of proinflammatory mediators : nitric oxide, interleukin 1 (IL-1), IL-6, IL-8, and tumor necrosis factor α. |
| General description: | FliC (flagellin) is the subunit protein which polymerizes to form the filaments of bacterial flagella. The protein has four-domains (D0, D1, D2, D3). |
| General description: | The encoded flagellin filament contains 55 kDa monomers. |
| Physical form: | Lyophilized from PBS. |
| Reconstitution: | Reconstitute with 100 μL deionized water. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |