IL-35 (mouse): FC (human)
SIGMA/SRP8053 - recombinant, expressed in CHO cells
Synonym: Interleukin-35; T-cell immunoglobulin and mucin domain-containing protein 4
Product Type: Chemical
assay | ≥98% (SDS-PAGE) |
biological source | human |
mouse | |
color | white |
form | lyophilized |
impurities | <0.06 EU/μg endotoxin, tested |
mol wt | monomer 23 kDa by calculation (IL-12a (p35)) |
monomer 46 kDa by calculation (Ebi3) | |
packaging | pkg of 5 μg |
recombinant | expressed in CHO cells |
shipped in | wet ice |
storage temp. | −20°C |
UniProt accession no. | O35228 |
P43431 |
Application: | IL-35 has been used to study its immunosuppressive effect on acute graft-versus-host disease in a mouse model. |
Biochem/physiol Actions: | Regulatory T cells are essential for maintaining self tolerance and preventing autoimmunity, and IL-35 is identified as a molecule that mediates the immune suppression function of Treg-cell. As an inhibitory cytokine, IL-35 induces proliferation of Treg-cell populations but suppresses Th17 cell development. Studies in mice show the absence of either IL-35 chain from Treg-cell reduces the cells′ ability to suppress inflammation using an experimental model for inflammatory bowel disease. IL-35 is suggested as a potential target of immunotherapy. |
General description: | Interleukin-35 (IL-35) is a novel IL-12 family cytokine produced by regulatory T cells (Treg) but not by resting or activated effector T cells (Teff). IL-35 is a heterodimeric protein composed of EBI3 (Epstein-Barr-Virus-induc |
Other Notes: | The mouse IL-35 complex composed of the Ebi3 subunit (aa 23-228) and the IL-12a (p35) subunit (aa 23-215) is fused through a polypeptide linker to the Fc region of human IgG1. |
Physical form: | Lyophilized from 0.2 μm-filtered solution in PBS. |
Reconstitution: | Reconstitute at 100 μg/mL in sterile PBS. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 2 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥98% (SDS-PAGE) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |