IL-35 (mouse): FC (human)
SIGMA/SRP8053 - recombinant, expressed in CHO cells
Synonym: Interleukin-35; T-cell immunoglobulin and mucin domain-containing protein 4
Product Type: Chemical
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| assay | ≥98% (SDS-PAGE) |
| biological source | human |
| mouse | |
| color | white |
| form | lyophilized |
| impurities | <0.06 EU/μg endotoxin, tested |
| mol wt | monomer 23 kDa by calculation (IL-12a (p35)) |
| monomer 46 kDa by calculation (Ebi3) | |
| packaging | pkg of 5 μg |
| recombinant | expressed in CHO cells |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | activity assay: suitable |
| UniProt accession no. | O35228   |
| P43431 |
| Application: | IL-35 has been used to study its immunosuppressive effect on acute graft-versus-host disease in a mouse model.Ithas also been used to study its effects on the differentiation of granulocytemacrophage-col |
| Biochem/physiol Actions: | Regulatory T cells are essential for maintaining self tolerance and preventing autoimmunity, and IL-35 is identified as a molecule that mediates the immune suppression function of Treg-cell. As an inhibitory cytokine, IL-35 induces proliferation of Treg-cell populations but suppresses Th17 cell development. Studies in mice show the absence of either IL-35 chain from Treg-cell reduces the cells′ ability to suppress inflammation using an experimental model for inflammatory bowel disease. IL-35 is suggested as a potential target of immunotherapy.IL-35 is produced inresponse to agonists of toll-like receptors 3 and 4 (TLR 3 & 4) and interferon γ (IFN-γ). It is secreted mainly by activated B cells, CD4+ Foxp3+ regulatoryT cells (Tregs), and at a lower concentration by monocytes, smooth muscle cells, and endothelial cells. |
| General description: | Research area: IMMUNO AND CKS. Interleukin-35 (IL-35) is a novel IL-12 family cytokine produced by regulatory T cells (Treg) but not by resting or activated effector T cells (Teff). IL-35 is a heterodimeric protein composed of EBI3 (Epstein-Barr-Virus-induc |
| Other Notes: | The mouse IL-35 complex composed of the Ebi3 subunit (aa 23-228) and the IL-12a (p35) subunit (aa 23-215) is fused through a polypeptide linker to the Fc region of human IgG1. |
| Physical form: | Lyophilized from 0.2 μm-filtered solution in PBS. |
| Preparation Note: | Reconstitute at 100 μg/mL in sterile PBS. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |