Prolactin human
SIGMA/SRP9000 - human, recombinant, expressed in HEK 293 cells
Synonym: PRL
Product Type: Chemical
Purity
The purity of human, recombinant Prolactin expressed in HEK 293 cells (Cat. No. SRP9000) was assessed on SDS-PAGE under reducing conditions (+DTT) followed by InstantBlue™ staining (Cat. No. ISB1L). (Representative result.)
Lanes
1. 1.25 μg/lane
2. 2 μg/lane
The purity of human, recombinant Prolactin expressed in HEK 293 cells (Cat. No. SRP9000) was assessed on SDS-PAGE under reducing conditions (+DTT) followed by InstantBlue™ staining (Cat. No. ISB1L). (Representative result.)
Lanes
1. 1.25 μg/lane
2. 2 μg/lane
Deglycosylation
Human, recombinant Prolactin expressed in HEK 293 cells (Cat. No. SRP9000) (lanes 3-4) and Prolactin expressed in E. coli (lanes 1-2) were either treated with PNGase F (lanes 2, 4) or untreated (lanes 1, 3), separated on SDS-PAGE followed by InstantBlue™ staining (Cat. No. ISB1L). (Representative result)
Lanes
1. E. coli Prolactin - PNGase F
2. E. coli Prolactin + PNGase F
3. HEK 293 Prolactin - PNGase F
4. HEK 293 Prolactin + PNGase F
Human, recombinant Prolactin expressed in HEK 293 cells (Cat. No. SRP9000) (lanes 3-4) and Prolactin expressed in E. coli (lanes 1-2) were either treated with PNGase F (lanes 2, 4) or untreated (lanes 1, 3), separated on SDS-PAGE followed by InstantBlue™ staining (Cat. No. ISB1L). (Representative result)
Lanes
1. E. coli Prolactin - PNGase F
2. E. coli Prolactin + PNGase F
3. HEK 293 Prolactin - PNGase F
4. HEK 293 Prolactin + PNGase F
Bioassay The biological activity of human, recombinant Prolactin expressed in HEK 293 cells (Cat.No. SRP9000) was measured in a cell proliferation assay using Nb2-11 cells. The EC50 is defined as the effective concentration that elicits a 50% increase in cell growth in a cell-based bioassay. (Representative result.)
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | liquid |
| impurities | ≤1 EU/μg protein Endotoxin level |
| mol wt | 23 kDa |
| potency | ≤2 ng/mL Nb2-11 cells proliferation EC50 |
| Quality Level | 200  |
| recombinant | expressed in HEK 293 cells |
| shelf life | 2 yr |
| sterility | non-sterile |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Prolactin human has been used: • in in vitro experiments to examine its effects in sleep-like concentrations on T-cell migration • to study its effects on claudin 2 (CLDN2) expression in the Caco-2 intestinal epithelial cell model • in microplate assays to demonstrate the specificity of the antibodies for vasoinhibin |
| Application: | Prolactin is glycosylated. |
| Biochem/physiol Actions: | Glycosylated human prolactin (G-hPRL) was first isolated and purified from human pituitaries by Lewis et al., with an estimated molecular mass of 25,000 Da and an immunological and biological activity of 25–50% that of non-glycosylated hPRL. The presence of a unique and partially occupied glycosylation site in Asn-31 in human, monkey, ovine, porcine, dromedary, equine and whale PRL makes it an ideal model of glycosylation for N-glycan studies since it exhibits the simplest type of glycosylation macroheterogeneity, with an occupancy range of 10-30% of G-hPRL relative to the total hPRL of either pituitary or recombinant origin. It has been postulated that hPRL glycosylation might possibly modulate the bioactivity of the circulating pool of the hormone, perhaps by selectively down regulating PRL action at individual target tissues. |
| Biochem/physiol Actions: | Prolactin is recognized in breast milk and can enhance Ca2+ absorption through both transcellular and paracellular pathways in the small and large intestine. It is crucial for lactation and reproduction and is demonstrated to have numerous effects on growth, development, metabolism, immunoregulation, and protection. The prolactin signaling pathway begins with the binding of prolactin to the prolactin receptor (PRLR). |
| General description: | Prolactin is a lactogenic hormone that plays a role in breast cancer, regulation of reproductive function, and immunoregulation. The prolactin cDNA encodes a 227 amino acid residue protein with a putative 28 amino residue signal peptide. Removal of the signal peptide results in the mature hormone corresponding to amino acids 29-227 of natural prolactin. There are several natural occurring molecular forms of prolactin, including a monomer, a non-glycosylated form, and a glycosylated form. |
| General description: | Prolactin is manufactured using an all-human production system, with full chemically defined ingredients and with no serum. It is therefore completely animal- and xeno-component free. |
| General description: | Research Area: IMMUNO AND CKS Prolactin (PRL) is a multifunctional polypeptide hormone primarily produced by the lactotrophic cells of the anterior pituitary gland in vertebrates. |
| Physical form: | This product is supplied as a solution in 0.2 μm filtered phosphate buffered saline with no additives or carrier proteins. It is aseptically filled. |
| Preparation Note: | Briefly centrifuge the vial before opening. After initial thawing it is recommended to store the protein in working aliquots at -20°C. The product can be diluted in PBS. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H360 |
| Precautionary statements | P201 - P308 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |