Leukemia Inhibitory Factor
SIGMA/SRP9001 - human, recombinant, expressed in HEK 293 cells
Synonym: Differentiation-
Product Type: Chemical
Purity
The purity of human, recombinant Leukemia Inhibitory Factor expressed in HEK 293 cells (Cat. No. SRP9001) was assessed on SDS-PAGE (1.25 μg/lane) followed by InstantBlue™ staining (Cat. No. ISB1L). (Representative result)
The purity of human, recombinant Leukemia Inhibitory Factor expressed in HEK 293 cells (Cat. No. SRP9001) was assessed on SDS-PAGE (1.25 μg/lane) followed by InstantBlue™ staining (Cat. No. ISB1L). (Representative result)
Deglycosylation
Human, recombinant Leukemia Inhibitory Factor expressed in HEK 293 cells (Cat. No. SRP9001) was treated with PNGase F and separated on SDS-PAGE followed by InstantBlue™ staining (Cat. No. ISB1L). (Representative result)
Lanes
1. HEK 293 LIF - PNGase F
2. HEK 293 LIF + PNGase F
Human, recombinant Leukemia Inhibitory Factor expressed in HEK 293 cells (Cat. No. SRP9001) was treated with PNGase F and separated on SDS-PAGE followed by InstantBlue™ staining (Cat. No. ISB1L). (Representative result)
Lanes
1. HEK 293 LIF - PNGase F
2. HEK 293 LIF + PNGase F
Bioassay The biological activity of human, recombinant Leukemia Inhibitory Factor expressed in HEK 293 cells (Cat. No. SRP9001) was measured in a cell citotoxicity assay using M1 cells. The EC50 is defined as the effective concentration that elicits a 50% inhibition in cell growth in a cell-based bioassay. (Representative result)
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | liquid |
| impurities | ≤1 EU/μg protein Endotoxin level |
| mol wt | 20 kDa |
| packaging | pkg of 10 μg |
| potency | ≤0.5 ng/mL M1 cells proliferation inhibition EC50 |
| Quality Level | 200  |
| recombinant | expressed in HEK 293 cells |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P15018  |
| Application: | Leukemia inhibitory factor (LIF) has been used to evaluate its effect on embryo development and aneuploidy in matured (IVM) human oocytes, in vitro. |
| Application: | One of the main uses of LIF in research is in mouse embryonic stem cell culture maintenance. LIF is necessary to maintain the stem cells in an undifferentiated state while retaining their proliferative potential or pluripotency. Removal of LIF pushes stem cells toward differentiation. |
| Biochem/physiol Actions: | Leukemia inhibitory factor (LIF) suppresses the differentiation of myoblasts. It also inhibits the activation of caspase-3 and blocks the reduction in Bcl-xL (B cell lymphoma) levels, thus, preventing doxorubicin-induced apoptosis. Along with transforming growth factor β2 (TGFβ2)/fibroblast growth factor 2 (FGF2), LIF regulates nephrogenesis. It facilitates tubular regeneration following acute kidney failure. It plays a crucial role in neuroprotective pathways induced by preconditioning-induced oxidative stress. LIF is capable of maintaining the totipotency of embryonic stem cells. It is also involved in controlling the differentiation of embryonic stem cells, hematopoietic and neuronal cells. |
| General description: | Leukemia Inhibitory Factor (LIF) gene is mapped on the human chromosome at 22q12.2. |
| General description: | Leukemia inhibitory factor (LIF) is a secreted pleiotropic glycoprotein which is a member of the gp130 signaling of interleukin-6 (IL-6) family cytokines. This protein was originally identified as a protein that prevented the growth of a leukemic cell line. |
| General description: | LIF is manufactured using an all-human production system, with no serum. |
| Physical form: | This product is supplied as a solution in 0.2 μm filtered phosphate buffered saline with no additives or carrier proteins. It is aseptically filled. |
| Preparation Note: | Briefly centrifuge the vial before opening. After initial thawing it is recommended to store the protein in working aliquots at -20°C. The product can be diluted in PBS. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |