Spectrum™ Plant Total RNA Kit
SIGMA/STRN250 - sufficient for 250 purifications
Synonym: Plant total RNA extraction kit; Plant total RNA isolation kit; Plant total RNA purification kit
Product Type: Chemical
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
| greener alternative category | Aligned  , |
| greener alternative product characteristics | Designing Safer Chemicals Learn more about the Principles of Green Chemistry . |
| Quality Level | 200 |
| storage temp. | 15-25°C |
| sustainability | Greener Alternative Product |
| technique(s) | RNA purification: suitable |
| usage | sufficient for 250 purifications |
| Application: | Spectrum™Plant Total RNA Kit has been used: • to extract RNA from the material ground in liquid nitrogen • to isolate total RNA from mutant and wt (wild type) plantlets • to extract total RNA from leaf samples • to extract RNA from all plant tissues |
| Features and Benefits: | • Yields up to 60 μg of pure concentrated RNA per prep • Efficient protocol allows for RNA purification in 30 min or less • Specially designed for research with difficult plant tissues |
| General description: | Click here to view the kit protocol |
| General description: | The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg. After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants. Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry. |
| Legal Information: | Spectrum is a trademark of Sigma-Aldrich Co. LLC |
| Symbol |     GHS05,GHS06,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H301 + H331 - H310 - H315 - H317 - H318 - H361d - H373 - H410 |
| Precautionary statements | P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338 |
| Hazard Codes | T,N |
| Risk Statements | 23/24/25-37/38-41-43-48/22-50/53 |
| Safety Statements | 26-36/37/39-45-61 |
| RIDADR | UN 3316 9 |
| WGK Germany | WGK 3 |
| Storage Temp. | 15-25°C |
| UNSPSC | 41105501 |
| Components | 2-Mercaptoethanol, Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration) 2; Binding Solution; Lysis Solution; Wash Solution 1 |