Anti-β-Tubulin antibody, Mouse monoclonal
SIGMA/T0198 - clone D66, purified from hybridoma cell culture
Synonym: Anti-Tubulin beta-1 chain
MDL Number: MFCD03792414
Product Type: Chemical
Western Blotting JOURNAL CITATION: Dengue virus co-opts UBR4 to degrade STAT2 and antagonize type I interferon signaling. By: Morrison, J., Laurent-Rolle, M., et al. in PLoS Pathog, 2013. PubMed ID: 23555265 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.ppat.1003265), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Dengue virus co-opts UBR4 to degrade STAT2 and antagonize type I interferon signaling. By: Morrison, J., Laurent-Rolle, M., et al. in PLoS Pathog, 2013. PubMed ID: 23555265 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.ppat.1003265), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Regulation of p14ARF expression by miR-24: a potential mechanism compromising the p53 response during retinoblastoma development. By: To, K. H., Pajovic, S., et al. in BMC Cancer, 2012. PubMed ID: 22336108 Image collected and cropped by CiteAb from the following publication, (http://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-12-69), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence SH-SY-5Y cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 0.5 μg/mL Monoclonal Anti-β-Tubulin, Clone: D66 (Cat. No. T0198). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Total cell extract of PC-12 was separated on SDS-PAGE and blotted with Monoclonal Anti-β-tubulin (Cat. No. T0198). The antibody was developed with Goat Anti-Mouse IgG, alkaline phosphatase conjugate (Cat. No. A2179) and a chemiluminescent substrate. Lanes 1. 0.5 μg/mL antibody 2. 1 μg/mL antibody
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-β-Tubulin, Clone: D66 (Cat. No. T0198). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa; 2. U87; 3. P19; 4. Neuro-2a; 5. PC-12; 6. COS-7; 7. CHO; 8. MDBK; 9. MDCK
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | D66, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~50 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, bovine, chicken, rat, canine, Chlamydomonas reinhardtii, sea urchin (Lytechinus pictus and Paracentrotus lividus), mouse, hamster, Oxyrrhis marina (dinoflagellate), rabbit, monkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable (3% paraformaldehyde-0.5% Triton X-100 and methanol-acetone) |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 0.5-1.0 μg/mL using whole extract of cultured rat adrenal pheochromocytoma (PC-12) cells | |
| UniProt accession no. | Q9H4B7  |
| Application: | Can be used in studies involving the inhibition of flagellar motility in permeabilized sperm models. |
| Application: | Monoclonal Anti-β-Tubulin antibody produced in mouse has also been used in immunofluorescence assay and enzyme linked immunosorbent assay (ELISA). |
| Application: | Monoclonal Anti-β-tubulin antibody produced in mouse has been used in western blotting and immunocytochemistry. |
| Biochem/physiol Actions: | Mutation in the gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. B2702 peptide binds to β- tubulin and inhibits natural killer (NK) cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. β-1 tubulin, also known as class VI or TUBB1, plays a vital role in platelet production, and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP). |
| Biochem/physiol Actions: | Recognizes all mouse brain β-tubulin isoforms (β and β′ subpopulations), and sea urchin β2-tubulin. The epitope recognized by the antibody is localized in the C-terminal region of β-tubulin (amino acids 427-432 in sea urchin β2-tubulin). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | β-Tubulin belongs to the subfamily of tubulins, which is the major building block of microtubules. β-tubulin has a molecular weight of 55kDa. β−Tubulin structure is characterized with core of two β -sheets enclosed by a-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site, and a C-terminal domain that contains the binding surface for molecular motor proteins. Human β-tubulin consists of seven isoforms (β I (class I), β II (class II), β III (class III), β IVa (class IVa), β IVb (class IVb), β V (class V), and β VI (class VI)). |
| General description: | Monoclonal Anti-β-Tubulin (mouse IgG1 isotype) is derived from the D66 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a sea urchin (Lytechinus pictus) sperm axonemal proteins. |
| Immunogen: | sea urchin (Lytechinus pictus) sperm axonemal proteins. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |