Tau-412 human
SIGMA/T0326 - recombinant, expressed in E. coli, ≥90% (SDS-PAGE), lyophilized powder
MDL Number: MFCD07785734
Product Type: Chemical
application(s) | cell analysis |
assay | ≥90% (SDS-PAGE) |
biological source | human |
form | lyophilized powder |
mol wt | 42.9 kDa |
Quality Level | 200 |
recombinant | expressed in E. coli |
shipped in | dry ice |
storage temp. | −70°C |
UniProt accession no. | P10636 |
Biochem/physiol Actions: | Isoform of Tau, variant 1N4R, having 4 microtubule binding repeats (R) and one amino terminal insert (N). |
Biochem/physiol Actions: | Tau promotes assembly and stabilizes neuronal microtubules under normal physiological conditions. Under pathological conditions Tau can also undergo modifications such as hyperphosphorylation, which can result in the generation of aberrant aggregates such as found in neurofibrillary tangles in Alzheimer′s disease. Tau-412 human is purified without any acid treatment and is suitable for promoting microtubule assembly, and for hyperphosphorylation-indu |
General description: | Tau-412 human, (1N4R) recombinant, is one of the isoforms of Tau and lacks a projection domain region. The isoforms of tau are generated by alternate splicing. The N terminal region of tau is acidic and is separated from the C-terminal tubulin-binding region by a central proline-rich region. The isoforms differ in amino-terminal N1 and N2 regions as well as binding repeats at the tubulin region. Tau belongs to the family of neuronal microtubule-associated proteins. |
Reconstitution: | Lyophilized from MES, pH 6.8, containing NaCl and EGTA. When reconstituted in water to a protein concentration of 1 mg/mL, the resulting buffer will have ~50 mM MES, pH 6.8, 100 mM NaCl, and 0.5 mM EGTA. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥90% (SDS-PAGE) |
Storage Temp. | −70°C |
UNSPSC | 12352200 |