Tricine
SIGMA/T0377 - ≥99% (titration)
Synonym: N-
CAS Number: 5704-04-1
Empirical Formula (Hill Notation): C6H13NO5
Molecular Weight: 179.17
EC Number: 227-193-6
MDL Number: MFCD00004277
Linear Formula: (HOCH2)3CNHCH2CO2H
Product Type: Chemical
application(s) | diagnostic assay manufacturing |
assay | ≥99% (titration) |
form | crystalline powder |
InChI | 1S/C6H13NO5/c8-2-6(3-9,4- |
InChI key | SEQKRHFRPICQDD-UHFFFAOYSA |
mp | 187 °C |
pKa (25 °C) | 8.1 |
Quality Level | 400 |
SMILES string | OCC(CO)(CO)NCC(O)=O |
solubility | water: 0.25 g/mL, clear, colorless |
useful pH range | 7.4-8.8 |
Application: | Buffer component for separation of low molecular weight peptides. |
Application: | Tricine has been used: • to prevent precipitation of salts during autoclaving of Emiliania huxleyi cultures • as a component of buffer A for the homogenization of samples like Caenorhabditis elegans, Drosophila, and plants • as a component of fresh assay buffer to measure serum melatonin by radioimmunoassay |
General description: | Tricine is the most frequently used electrophoresis buffer. It is also useful for the resuspension of cell pellets. Tricine functions as a trailing ion and aids the resolution of small proteins at lower acrylamide concentrations than in glycine-sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) systems. It has a lower negative charge than glycine, which helps it migrate faster. Tricine has a high ionic strength that allows increased ion movement and less protein movement. This in turn leads to the separation of low molecular weight proteins in lower percent acrylamide gels. |
Packaging: | 1 kg in poly bottle |
Packaging: | 25, 100, 250 g in poly bottle |
Packaging: | 5, 25 kg in poly drum |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥99% (titration) |
mp | 187 °C |
UNSPSC | 12161700 |