Tricine
SIGMA/T0377 - ≥99% (titration)
Synonym: N-
CAS Number: 5704-04-1
Empirical Formula (Hill Notation): C6H13NO5
Molecular Weight: 179.17
EC Number: 227-193-6
MDL Number: MFCD00004277
Linear Formula: (HOCH2)3CNHCH2CO2H
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T0377-100G
This image depicts SKU: T0377-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T0377-25G
This image depicts SKU: T0377-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T0377-250G
This image depicts SKU: T0377-250G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T0377-1KG
This image depicts SKU: T0377-1KG
| application(s) | diagnostic assay manufacturing |
| assay | ≥99% (titration) |
| form | crystalline powder |
| impurities | ≤5 ppm Heavy Metals (as Lead) |
| InChI | 1S/C6H13NO5/c8-2-6(3-9,4- |
| InChI key | SEQKRHFRPICQDD-UHFFFAOYSA |
| mp | 187 °C |
| pKa (25 °C) | 8.1 |
| Quality Level | 400  |
| SMILES string | OCC(CO)(CO)NCC(O)=O |
| solubility | water: 0.25 g/mL, clear, colorless |
| suitability | suitable for electrophoresis |
| technique(s) | SDS-PAGE: suitable |
| useful pH range | 7.4-8.8 |
| Application: | Buffer component for separation of low molecular weight peptides. |
| Application: | Tricine has been used: • to prevent precipitation of salts during autoclaving of Emiliania huxleyi cultures • as a component of buffer A for the homogenization of samples like Caenorhabditis elegans, Drosophila, and plants • as a component of fresh assay buffer to measure serum melatonin by radioimmunoassay |
| General description: | Tricine is the most frequently used electrophoresis buffer. It is also useful for the resuspension of cell pellets. Tricine functions as a trailing ion and aids the resolution of small proteins at lower acrylamide concentrations than in glycine-sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) systems. It has a lower negative charge than glycine, which helps it migrate faster. Tricine has a high ionic strength that allows increased ion movement and less protein movement. This in turn leads to the separation of low molecular weight proteins in lower percent acrylamide gels. |
| Packaging: | 1 kg in poly bottle |
| Packaging: | 25, 100, 250 g in poly bottle |
| Packaging: | 5, 25 kg in poly drum |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99% (titration) |
| mp | 187 °C |
| UNSPSC | 12161700 |