Monoclonal Anti-Transforming Growth Factor-β1 antibody produced in mouse
SIGMA/T0438 - clone 9016, purified immunoglobulin, lyophilized powder
Synonym: Anti-TGF-β1; CEDLAP; DPD1; latency-associated peptide
MDL Number: MFCD00802737
Product Type: Chemical
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | 9016, monoclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| isotype | IgG1 |
| Quality Level | 200  |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| technique(s) | ELISA: suitable |
| flow cytometry: suitable | |
| immunocytochemistry: suitable | |
| immunohistochemistry: suitable | |
| neutralization: suitable | |
| western blot: suitable | |
| UniProt accession no. | P01137  |
| Application: | Recommended concentrations: Western Blot 1 ug/mL (under non-reducing conditions only) Immunohistochemistry 3-25 μg/mL Flow Cytometry 2.5 μg/106 cells ELISA capture 2-8 μg/mL ELISA detection 0.1-0.4 μg/mL Neutrilization 0.3-1.0 μg/mL |
| Application: | Monoclonal Anti-Transforming Growth Factor-β1 antibody produced in mouse has been used in: • immunohistochemical detection of TGFβ1 in smooth muscle(2) and liver sections • western blotting and ELISA of brain specimens • immunoblot detection of TGFβ1 in macrophages |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Transforming growth factor-β (TGF-β) belongs to the TGF-β superfamily of secreted proteins. TGF-β 1 exists as three isoforms TGF-β 1, TGF-β 2 and TGF-β 3. The TGF-β family is involved in a variety of signaling pathways that involve serine/threonine protein kinases. TGF-β acts through binding to three transmembrane receptors in mammals, type I, type II and type III receptors. The important pathways regulated by TGF-β are the SMAD, BMP, MAPK and PI3K/Akt pathways. TGF-β 1 is involved in cell and tissue differentiation, proliferation, inflammation, migration of monocytes, neutrophils and chemotaxis. Deregulation of TGF-β signaling has been implicated in many inflammation and autoimmune diseases. Monoclonal Anti-Transforming Growth Factor-β 1 antibody specifically reacts with human, mouse and rat TGF-β 1. The antibody shows less than 2% cross reactivity with TGF-β3 and TGF-β5 in ELISA. |
| General description: | Transforming Growth Factor-β1 (TGFβ1) is mapped to human chromosome 19q13.2. |
| Immunogen: | Chinese hamster ovary cell line CHO-derived recombinant TGF- beta 1 and latent TGF- beta 1 |
| Other Notes: | Protein A or G purified from hybridoma culture supernatant. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates. |
| Reconstitution: | Reconstitute at 0.5 mg/mL in sterile PBS. |
| Specificity: | By capture ELISA, <2% cross-reactivity is observed with TGF-β3 and TGF-β5. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |