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3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes

SIGMA/T0565 - ready to use solution

Synonym: TMB membrane substrate

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-T0565-100ML 100 mL
$133.00
1/EA
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Sigma offers specially formulated TMB liquid substrate systems for ELISA requiring a soluble end-product and for western blotting procedures that require an insoluble end-product. TMB Liquid Substrate System for peroxidase-based ELISA procedures where the soluble blue end product can be read at 370 nm or 655 nm. The addition of an acidic stopping solution produces a yellow color that can be read at 450 nm. The Sigma TMB Substrate System for Membranes produces an insoluble, dark blue end-product, and is not recommended for ELISA or immunohistochemical applications.

 

form liquid
Quality Level 100 
storage temp. 2-8°C
Application: 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes has been used as a chromogenic substrate in western blot analysis. It has also been used as a liquid substrate in enzyme-linked immunosorbent assay (ELISA).
Application: 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes has been used in:
• enzyme-linked immune absorbent spot (ELISPOT) assay
• as a substrate for IgG peroxidase
• in the visualization of immunocomplexes

General description: 3,3′,5,5′-Tetramethylbenzidine (TMB) is a chromogenic substrate for horseradish peroxidase (HRP) conjugates. It develops a permanent, insoluble, dark blue reaction product and is useful in colorimetric quantification.
Physical form: Ready-to-use.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. 2-8°C
UNSPSC 12352204

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