holo-Transferrin human
SIGMA/T0665 - powder, BioReagent, suitable for cell culture, ≥97%
Synonym: Siderophilin, iron-saturated
CAS Number: 11096-37-0
EC Number: 234-318-8
MDL Number: MFCD00131337
Product Type: Chemical
assay | ≥97% |
biological source | human plasma |
description | Iron-saturated |
form | powder |
impurities | endotoxin, tested |
HIV and HBsAg, none detected | |
product line | BioReagent |
Quality Level | 200 ![]() |
shipped in | ambient |
solubility | H2O: 50 mg/mL |
storage temp. | 2-8°C |
technique(s) | cell culture | mammalian: suitable |
UniProt accession no. | P02787 ![]() ![]() |
Analysis Note: | Iron content by ICP reported on a lot-specific basis. |
Analysis Note: | Purity by agarose gel electrophoresis. |
Application: | holo-Transferrin human has been supplemented in Roswell Park Memorial Institute (RPMI) for proerythroblasts proliferation assay and also in culture media for inductively coupled plasma emission spectroscopy. |
Application: | Transferrin is the primary iron binding and cell delivery molecule present in serum. Serum-free cell culture systems require a delivery format for iron. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Human holo-transferrin is a high affinity transferrin that can be used with a wide range of cells types. Human holo-transferrin is loaded with iron prior to use and can be added directly to iron poor or iron-free cell culture media. |
Biochem/physiol Actions: | Provides iron to cells in a physiologically stable and safe form. |
Packaging: | 1 g in poly bottle |
Packaging: | 50, 100, 500 mg in poly bottle |
Preparation Note: | Heat treated at 60 °C (minimum) for a minimum of 10 hours. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Purity | ≥97% |
Storage Temp. | 2-8°C |
UNSPSC | 12352200 |