Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse
SIGMA/T0678 - clone WH-3, ascites fluid
Synonym: Anti-TPH
MDL Number: MFCD00282500
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded Human colon carcinoma with 1:100 Monoclonal Tryptophan Hydroxylase, Clone: WH-3 (Cat. No. T0678) using biotin/ExtrAvidin®-Peroxidase.
Immunoblotting Whole extract of MDBK cells was separated on SDS-PAGE and probed with Monoclonal Anti-Tryptophan Hydroxylase, Clone: WH-3 (Cat. No. T0678). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Antibody dilution: 1:500 2. Antibody dilution: 1:1,000 3. Negative control: without first antibody
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | WH-3, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG3 |
| mol wt | antigen 55 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, monkey, rabbit, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 1:1,000 using rabbit pineal gland extract | |
| UniProt accession no. | P17752  |
| Application: | Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse has been used in: • enzyme-linked immunosorbent assay (ELISA) • immunohistochemistry • immunoblot • immunocytochemistry |
| Biochem/physiol Actions: | The tryptophan hydroxylase 1 gene (TPH1) catalyzes the synthesis of 5-hydroxytryptophan, which is a precursor to the neurotransmitter serotonin. Variation in the gene expression affects prefrontal cortex activation during response inhibition. Mutation in the TPH1 gene leads to schizophrenia, irritable bowel syndrome (IBS) in women, suicidal behavior and depressive disorders. The encoded protein plays a vital role in regulation of cardiovascular function. |
| Biochem/physiol Actions: | This antibody reacts specifically with tryptophan hydroxylase. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Tryptophan Hydroxylase (mouse IgG3 isotype) is derived from the WH-3 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Tryptophan hydroxylase (TPH; |
| General description: | The tryptophan hydroxylase 1 gene (TPH1) is a 444 amino acid protein encoded by the gene spanning 29 kbp with11 exons on human chromosome 11p15.3-p14. The enzyme is expressed in the gut, spleen, thymus, pituitary and also in the pineal gland. |
| Immunogen: | recombinant rabbit tryptophan hydroxylase. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |