Anti-Transportin 1 antibody, Mouse monoclonal
SIGMA/T0825 - clone D45, purified from hybridoma cell culture
Synonym: Anti-IPO2; Anti-KPNB2; Anti-MIP; Anti-MIP1; Anti-TRN
MDL Number: MFCD06202150
Product Type: Chemical
Immunoblotting Nuclear extract of HeLa cells was separated on SDS-PAGE and probed with Monoclonal Anti-Transportin 1, Clone: D45 (Cat. No. T0825). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A9917) and a chemiluminescent substrate. Lanes 1. 0.5 μg/mL 2. 0.25 μg/mL 3. 0.125 μg/mL 4. 0.0625 μg/mL 5. Negative control: without first antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | D45, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~90 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, canine, rat, human, bovine |
| storage temp. | −20°C |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| microarray: suitable | |
| western blot: 0.1-0.2 μg/mL using HeLa nuclear extract | |
| UniProt accession no. | Q92973  |
| Application: | Monoclonal Anti-Transportin 1 antibody produced in mouse has been used in western blotting, immunofluorescence, and immunostaining. |
| Biochem/physiol Actions: | Transportin 1 (TNPO1 or TRN1) recognizes the nuclear localization signals (NLS) and binds to the nuclear pore complex (NPC) to facilitate the transport of substances from cytoplasm to nucleus. TRN1 transports viral, and ribosomal proteins with the aid of RanGTP (Ras associated nuclear protein-GTP). TRN1 is also known to regulate the key events in the cell cycle assembly like nuclear membrane and nuclear pore. Mutations in TRN1 might cause amyotrophic lateral sclerosis due to mislocalisation of FUS (fused in sarcoma) protein. TRN1 is associated with regulation of circadian rhythm through nuclear localization of PER1 (period circadian protein homolog 1) protein. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Transportin 1 (TNPO1 or TRN1) also known as karyopherin-β2, is a nuclear import protein and belongs to importin-β family. In the human chromosome the TNPO1 gene is localized on 5q13.2.(10) |
| Immunogen: | recombinant human transportin 1. |
| Packaging: | 200 μL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| Target description: | Transportin 1 encodes the beta subunit of the karyopherin receptor complex which interacts with nuclear localization signals to target nuclear proteins to the nucleus. The karyopherin receptor complex is a heterodimer of an alpha subunit which recognizes |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |