Thioredoxin from Escherichia coli
SIGMA/T0910 - recombinant, expressed in E. coli, essentially salt-free, lyophilized powder, ≥3 units/mg protein
CAS Number: 52500-60-4
MDL Number: MFCD00132423
Product Type: Chemical
SDS-PAGE Thioredoxin from Escherichia coli (Cat. No. T0910) was assessed on SDS-PAGE.
Unit Definition 1 unit of thioredoxin activity causes an increase of 1.0 OD650 per minute at 25C at pH 7.0 in presence of 1mM DDT. DTT-(SH)2 = DL - Dithiothreitol Insulin-S2 = Insulin (native disulfide bridge) Insulin-(SH)2 = Insulin (reduced disulfides) DTT-S2 = DL - Dithiothreitol (disulfide bridge)
| application(s) | cell analysis |
| assay | ≥90% (SDS-PAGE) |
| biological source | Escherichia coli |
| form | essentially salt-free, lyophilized powder |
| mol wt | 11.7 kDa |
| Protein ID accession no. | M26133   |
| Quality Level | 200 |
| recombinant | expressed in E. coli |
| solubility | water: 1.90-2.10 mg/mL, clear, colorless |
| specific activity | ≥3 units/mg protein |
| storage temp. | −20°C |
| technique(s) | activity assay: suitable |
| UniProt accession no. | P0AA25 |
| Application: | Thioredoxin from Escherichia coli has been used in thioredoxin reductase assay, peroxidase-coupled thioredoxin system assay and peroxiredoxin assay. It has also been used to study its effects on photoreceptor death in a mouse model of retinitis pigmentosa (RP) and its potential to modify retinal oxidative stress, glial changes, and vascular changes during the progression of the disease. |
| Biochem/physiol Actions: | Mediates the reduction of disulfide bonds in proteins. |
| Biochem/physiol Actions: | Thioredoxin from Escherichia coli catalyzes REDOX reactions and has a role in various biological processes. It modulates the structure and activity of many proteins. The enzyme thioredoxin reductase catalyzes the reduction of thioredoxin. Thioredoxin also has a role in oxidative stress response.Thioredoxin, known as both adult T cell leukemia-derived factor (ADF) and a cellular redox enzyme, functions as a cytokine or chemokine, facilitating cell-to-cell communication. Additionally, it plays a role in regulating cell growth and apoptosis. |
| General description: | Research area: Cell Signaling Thioredoxin from Escherichia coli is a monomeric, globular protein. It has a central β-sheet surrounded by α-helices. |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Other Notes: | GenBank® Accession Number M26133. |
| Other Notes: | One unit will cause a ΔA650 of 1.0 in 1 min at 25 °C in the insulin reduction assay. |
| Packaging: | 1 mg in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| activity | specific activity: ≥3 units/mg protein |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |