Anti-γ-Tubulin (QG-17) antibody produced in rabbit
SIGMA/T0950 - affinity isolated antibody, buffered aqueous solution
MDL Number: MFCD06411817
Product Type: Chemical
Immunoblotting
Schneider’s drosophila (Dr.M. (2), SL2) cell line whole cell extract was separated on SDS-PAGE and blotted with Rabbit Anti-γ-Tubulin (QG-17) antibody (Cat. No. T0950). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and chemiluminescent substrate.
Antibody dilution 1:4,000. Inhibition of staining with γ-Tubulin immunizing peptide (20 μg/mL, Lane B).
Schneider’s drosophila (Dr.M. (2), SL2) cell line whole cell extract was separated on SDS-PAGE and blotted with Rabbit Anti-γ-Tubulin (QG-17) antibody (Cat. No. T0950). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and chemiluminescent substrate.
Antibody dilution 1:4,000. Inhibition of staining with γ-Tubulin immunizing peptide (20 μg/mL, Lane B).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 48 kDa |
| shipped in | dry ice |
| species reactivity | Drosophila |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 0.2-0.4 μg/mL using whole cell extract of the Schneider′s Drosophila Line 2 [D.mel.(2), SL2] |
| Application: | Anti-γ-Tubulin (QG-17) antibody has been used in immunoblotting and immunostaining. |
| Biochem/physiol Actions: | γ-tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. In Aspergillus nidulans, γ-tubulin facilitates attachment of microtubules to the spindle pole body, nuclear division and microtubule assembly. Ubiquitination of γ-tubulin by breast cancer 1 protein (BRCA1) is a crucial step in the regulation of centrosome number. Overexpression of γ-tubulin is observed in lung cancer. Tubulin γ 2 (TUBG2) plays a vital role in cell growth. Aberration in the γ-tubulin gene alters microtubule assembly. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas. |
| Biochem/physiol Actions: | The sequence is highly specific for Drosophila γ-tubulin and is not found in γ-tubulin of other species or other members of the tubulin family such as α-, β-, δ-, and ε-tubulins. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | γ-Tubulin (48 kDa) is a widely expressed and highly conserved protein within the microtubule organizing centers (MTOCs) or centrosome in eukaryotic cells. |
| General description: | γ-tubulin, mapped on human chromosome 17q21.2, codes for a member of the tubulin family. It is localized to the microtubule organizing centres. γ-tubulin consists of two isoforms tubulin γ 1 (TUBG1) and tubulin γ 2 (TUBG2) with 97.3% amino acid identity. In addition to these two isoforms, γ-tubulin pseudogene (TUBG1P) is also been identified on human chromosome 17. Human TUBG1 and TUBG2 transcripts are widely expressed in preimplantation embryos and the brain, respectively. γ-tubulin is a component of γ-tubulin ring complex (γ-TuRC), which has roughly the same diameter as a microtubule. |
| Immunogen: | synthetic peptide corresponding to the C-terminus of Drosophila γ-tubulin (amino acids 441-457 with N-terminal added cysteine-glycine), conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |