Anti-Tal (CS-15) antibody produced in rabbit
SIGMA/T1075 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-
MDL Number: MFCD06798551
Product Type: Chemical
Immunoblotting
Whole cell extracts of HEK293 cells transfected with human Tal (Lanes 1, 4), MDCK cells (Lane 2), and A431 cells (Lane 3) were separated on SDS-PAGE, blotted with Rabbit Anti-Tal (CS-15) (Cat. No. T1075) at a 1:2,000 dilution. Specificity of antibody is confirmed by inhibition of staining with the Tal (CS-15) immunizing peptide (20 μg/mL, Lane 4).
Whole cell extracts of HEK293 cells transfected with human Tal (Lanes 1, 4), MDCK cells (Lane 2), and A431 cells (Lane 3) were separated on SDS-PAGE, blotted with Rabbit Anti-Tal (CS-15) (Cat. No. T1075) at a 1:2,000 dilution. Specificity of antibody is confirmed by inhibition of staining with the Tal (CS-15) immunizing peptide (20 μg/mL, Lane 4).
Cytosolic fraction of rat brain was immunoprecipitated with the corresponding antibodies to Tal. IP samples were separated on SDS-PAGE and blotted with either Rabbit Anti-Tal (CS-15) (Cat No. T1075) or Anti-Tal (FQ-17) (Cat. NO. T1200) at a 1:2,000 dilution. The antibodies were developed with Goat Anti-Rabbit IgG-Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 84 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, canine, human, mouse |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): 5-10 μg using mouse brain S1 cytosolic fraction |
| microarray: suitable | |
| western blot: 0.1-0.2 μg/mL using whole extract of human epitheloid carcinoma HeLa cell line and also canine kidney MDCK cell line | |
| western blot: 0.2-0.4 μg/mL using whole extract of rat brain S1 cytosolic fraction | |
| UniProt accession no. | Q6UWE0  |
| Application: | Anti-Tal (CS-15) antibody produced in rabbit may be used in immunoblotting and immunoprecipitation. |
| Biochem/physiol Actions: | Tsg101-associated ligase (TAL) plays a key role in cell signaling, cell adhesion and regulates cargo sorting. Reduced expression of Tsg101 shunts active epidermal growth factor receptor (EGFRs) from the normal degradative pathway to a recycling route. Tal, which interacts with Tsg101 associates with and ubiquitylates Tsg101 in a manner that regulates receptor trafficking and budding of viral particles. Tal binds to Tsg101 in a bimodal mode, through an interaction of its CC domain with the steadiness box (SB) of Tsg101, and also via binding of the UEV domain of Tsg101 with two PTAP motifs just upstream of the C-terminal RING domain. Tal interaction with Tsg101 promotes multiple monoubiquitination of Tsg101, rather than polyubiquitination, which leads to the release of Tsg101 from membrane complexes and its inactivation. Mutations in the TAL gene is implicated in Charcot-Marie-Tooth (CMT) syndrome. |
| Disclaimer: | Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Tsg101-associated ligase (TAL), contains an N-terminal leucine-rich repeat (LRR), followed by an ezrin-radixin-moesin (ERM) domain, a coiled-coil (CC) region, a sterile alpha motif (SAM) domain and a C-terminal C3HC4-type RING finger domain. Also called leucine-rich repeat and sterile alpha motif-containing protein 1 (LRSAM1), TAL is an E3 ubiquitin ligase. TAL gene is mapped to human chromosome 9. |
| Immunogen: | synthetic peptide encoding amino acids 709-723 located near the C-terminus of human Tal, conjugated to KLH. This sequence is highly conserved in mouse and rat Tal (two amino acids substitution). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | Anti-Tal (CS-15) recognizes human, dog, rat, and mouse Tal (84 kDa, may appear as doublet). |
| Storage and Stability: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| WGK Germany | - |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |