Tissue Inhibitor of Metalloproteinase-2 human
SIGMA/T1077 - recombinant, expressed in CHO cells, >95% (SDS-PAGE), lyophilized powder
Synonym: TIMP-2
MDL Number: MFCD00285923
Product Type: Chemical
| assay | >95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| mol wt | apparent mol wt ~20 kDa |
| Quality Level | 100  |
| recombinant | expressed in CHO cells |
| storage temp. | −20°C |
| technique(s) | activity assay: suitable |
| inhibition assay: suitable | |
| UniProt accession no. | P16035  |
| Analysis Note: | The biological activity is measured by the ability to inhibit human MMP-2 hydrolysis of a fluorogenic MMP substrate. |
| Biochem/physiol Actions: | TIMP-2 has a greater binding efficiency to MMP-2 than the other MMPs. Although TIMP-2 is an inhibitor of MMP-2, it is also required at low concentrations for the activation of MMP-2. |
| Biochem/physiol Actions: | Tissue inhibitor of metalloproteinase-2 (TIMP-2), like other protease inhibitors, functions as a key modulator of extracellular matrix degradation during tissue development and remodeling. TIMP-2 can also act through a matrix metalloproteinase (MMP)-independent mechanism inhibiting endothelial cell proliferation in vitro. It demonstrates anti-angiogenic activities in vivo. TIMP-2 is a target gene of the microRNA-miR-22. |
| General description: | Tissue inhibitor of metalloproteinase-2 (TIMP-2) is an extracellular inhibitor of matrix metalloproteinases (MMPs). There are six conserved cysteine residues forming disulfide loops in its amino- and carboxy-terminal domains. The gene encoding this protein is localized on human chromosome 17q25. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in 25 mM Tris, and 150 mM sodium chloride, pH 7.5. |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |