Synonym: 5′-O-(4,4′-dimethoxytrityl)-2′-deoxythymidine-3′-O-[O-(2-cyanoethyl)-N,N′-diisopropylphosphoramidite]; 5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-deoxythymidine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite]; DMT-dT amidite; Thymidine, 5′-O-[bis(4-methoxyphenyl)phenylmethyl]-, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite]
CAS Number: 98796-51-1
Empirical Formula (Hill Notation): C40H49N4O8P
Molecular Weight: 744.81
MDL Number: MFCD00055063
Linear Formula: C40H49N4O8P
Product Type: Chemical
| λ |
conforms (UV/VIS Identity) |
| assay |
≥99% (31P-NMR) |
| |
≥99.0% (reversed phase HPLC) |
| biological source |
non-animal source (no BSE/TSE risk) |
| color |
white to off-white |
| compatibility |
configured for ÄKTA® and OligoPilot® |
| form |
powder or granules |
| impurities |
≤0.3 wt. % water content (Karl Fischer) |
| InChI |
1S/C40H49N4O8P/c1-27(2)44(28(3)4)53(50-23-11-22-41)52-35-24-37(43-25-29(5)38(45)42-39(43)46)51-36(35)26-49-40(30-12-9-8-10-13-30,31-14-18-33(47-6)19-15-31)32-16-20-34(48-7)21-17-32/h8-10,12-21,25,27-28,35-37H,11,23-24,26H2,1-7H3,(H,42,45,46)/t35-,36+,37+,53?/m0/s1 |
| InChI key |
UNOTXUFIWPRZJX-CEXSRUIHSA-N |
| nucleoside profile |
base: deoxythymidine
base protecting group: none
2' protecting group: none
5' protecting group: DMT
deprotection: fast/standard |
| product line |
Proligo Reagents |
| Quality Level |
200  |
| SMILES string |
COc1ccc(cc1)C(OC[C@H]2O[C@H](C[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)N3C=C(C)C(=O)NC3=O)(c4ccccc4)c5ccc(OC)cc5 |
| storage temp. |
2-8°C |
| technique(s) |
oligo synthesis: suitable |
| type |
for DNA synthesis |
| General description: |
Proligo′s DNA phosphoramidites lead to high-yield and high-quality oligonucleotides due to their high coupling efficiency. The deprotection step of automated oligonucleotide synthesis is integral to synthesis time and final product quality. Exocyclic amine functions are protected by a benzoyl group (dA(bz) and dC(bz)) or isobutyryl group (dG(ib)). Recommended cleavage and deprotection conditions are 8 hours at 55 °C or 24 hours at room temperature using concentrated ammonia solution, for standard base-protected oligonucleotides. DMT-dT Phosphoramidite belongs to ÄKTA® synthesizers. |
| Legal Information: |
ÄKTA is a registered trademark of Cytiva |
| Legal Information: |
OligoPilot is a registered trademark of Cytiva |
| RIDADR |
NONH for all modes of transport |
| WGK Germany |
WGK 1 |
| Flash Point(F) |
Not applicable |
| Flash Point(C) |
Not applicable |
| Purity |
≥99% (31P-NMR); ≥99.0% (reversed phase HPLC) |
| Storage Temp. |
2-8°C |
| UNSPSC |
41116107 |
