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DMT-dT Phosphoramidite

SIGMA/T111050 - configured for ÄKTA® and OligoPilot®

Synonym: 5′-O-(4,4′-dimethoxytrityl)-2′-deoxythymidine-3′-O-[O-(2-cyanoethyl)-N,N′-diisopropylphosphoramidite]; 5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-deoxythymidine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite]; DMT-dT amidite; Thymidine, 5′-O-[bis(4-methoxyphenyl)phenylmethyl]-, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite]

CAS Number: 98796-51-1
Empirical Formula (Hill Notation): C40H49N4O8P
Molecular Weight: 744.81
MDL Number: MFCD00055063
Linear Formula: C40H49N4O8P
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-T111050-5G 5 g
 $130.00
1/EA		 Add To Favorites
45-T111050-6X5G 5 g
 $737.00
6/EA		 Add To Favorites
45-T111050-10G 10 g
 $264.00
1/EA		 Add To Favorites

 

λ conforms (UV/VIS Identity)
assay ≥99% (31P-NMR)
  ≥99.0% (reversed phase HPLC)
biological source non-animal source (no BSE/TSE risk)
color white to off-white
compatibility configured for ÄKTA® and OligoPilot®
form powder or granules
impurities ≤0.3 wt. % water content (Karl Fischer)
InChI 1S/C40H49N4O8P/c1-27(2)44(28(3)4)53(50-23-11-22-41)52-35-24-37(43-25-29(5)38(45)42-39(43)46)51-36(35)26-49-40(30-12-9-8-10-13-30,31-14-18-33(47-6)19-15-31)32-16-20-34(48-7)21-17-32/h8-10,12-21,25,27-28,35-37H,11,23-24,26H2,1-7H3,(H,42,45,46)/t35-,36+,37+,53?/m0/s1
InChI key UNOTXUFIWPRZJX-CEXSRUIHSA-N
nucleoside profile base: deoxythymidine
base protecting group: none
2' protecting group: none
5' protecting group: DMT
deprotection: fast/standard
product line Proligo Reagents
Quality Level 200 
SMILES string COc1ccc(cc1)C(OC[C@H]2O[C@H](C[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)N3C=C(C)C(=O)NC3=O)(c4ccccc4)c5ccc(OC)cc5
storage temp. 2-8°C
technique(s) oligo synthesis: suitable
type for DNA synthesis
General description: Proligo′s DNA phosphoramidites lead to high-yield and high-quality oligonucleotides due to their high coupling efficiency. The deprotection step of automated oligonucleotide synthesis is integral to synthesis time and final product quality. Exocyclic amine functions are protected by a benzoyl group (dA(bz) and dC(bz)) or isobutyryl group (dG(ib)). Recommended cleavage and deprotection conditions are 8 hours at 55 °C or 24 hours at room temperature using concentrated ammonia solution, for standard base-protected oligonucleotides. DMT-dT Phosphoramidite belongs to ÄKTA® synthesizers.
Legal Information: ÄKTA is a registered trademark of Cytiva
Legal Information: OligoPilot is a registered trademark of Cytiva
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥99% (31P-NMR); ≥99.0% (reversed phase HPLC)
Storage Temp. 2-8°C
UNSPSC 12352200

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