Trizma® Pre-set crystals
SIGMA/T1194 - BioPerformance Certified, pH 8.5, average Mw 131.4
Synonym: Tris crystals
Product Type: Chemical
application(s) | diagnostic assay manufacturing |
form | crystalline powder |
grade | BioPerformance Certified |
impurities | DNase, RNase, Protease, NICKase, none detected |
endotoxin, tested | |
total aerobic microbial count, tested | |
≤0.05 A290 | |
≤0.5% water (Karl Fischer) | |
≤5 ppm Heavy metals (as Pb) | |
mol wt | average Mw 131.4 |
pH | 8.22 (37 °C, 0.1 M)(lit.) |
8.5 | |
8.50 (25 °C, 0.1 M)(lit.) | |
9.09 (5 °C, 0.1 M)(lit.) | |
Quality Level | 200 |
solubility | water: soluble, clear |
technique(s) | cell culture | mammalian: suitable |
useful pH range | 7.0-9.0 |
Application: | Open the foil pouch, dissolve contents in 900 mL of water; add additional water to a final volume of 1 liter to make a 0.1 M solution at a temperature of 25 °C. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units. Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature. From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C. From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C. If a different concentration is desired, note that there is a concentration effect on the pH of the solution. |
Application: | The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases. |
Legal Information: | Trizma is a registered trademark of Sigma-Aldrich Co. LLC |
Other Notes: | Certain pH electrodes do not give accurate results with Tris buffers. |
Other Notes: | This product is a pre-mixed combination of Trizma base and Trizma HCl in convenient foil pouches that can be used to prepare a common pH value for Tris buffer. |
Packaging: | In convenient foil pouches |
Preparation Note: | Trizma solutions can be autoclaved. |
Hazard Codes | Xi |
Risk Statements | 36/37/38 |
Safety Statements | 26 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
UNSPSC | 12161700 |