Monoclonal Anti-Tyrosine Hydroxylase antibody produced in mouse
SIGMA/T1299 - clone TH-2, ascites fluid
Synonym: Anti-DYT14; Anti-DYT5b; Anti-TYH
MDL Number: MFCD00213051
Product Type: Chemical
Western Blotting Whole extract of PC-12 cells was separated on SDS-PAGE and probed with Monoclonal anti Tyrosine Hydroxylase antibody, Clone: TH-2 (Cat. No. T1299). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1.1:10,000 antibody 2. Negative control: without first antibody
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | TH-2, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, monkey, guinea pig, rat, sheep, bovine, rabbit |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: suitable |
| immunoprecipitation (IP): suitable using native or SDS-denatured TH. | |
| western blot: 1:10,000 using extracts of cultured rat adrenal pheochromocytoma (PC12) cells. | |
| UniProt accession no. | P07101  |
| Application: | Monoclonal Anti-Tyrosine Hydroxylase antibody produced in mouse has also been used in • western blotting • indirect immunofluorescence • immunostaining • immunohistochemistry • enzyme linked immunosorbent assay (ELISA) • dot blot • immunoprecipitation |
| Application: | Monoclonal Anti-tyrosine hydroxylase antibody produced in mouse has been used in western blotting and immunocytochemistry. |
| Biochem/physiol Actions: | Tyrosine hydroxylase (TH) catalyzes the first rate limiting step in the biosynthesis of catecholamine neurotransmitter i.e. the conversion of L-tyrosine to L-dopa. Inhibition of TH by L-phenylalanine, might play a crucial role in phenylketonuria and block the synthesis of norepinephrine. Activity of TH can be regulated by phosphorylation. Decreased expression of TH is associated with various neuropsychiatric diseases such as schizophrenia and Parkinson’s disease (PD). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Tyrosine Hydroxylase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tyrosine hydroxylase (TH) RNA undergoes alternative splicing resulting in four isoforms of TH subunit protein in humans. The four human isoforms are TH-1 (55.6 kDa), TH-2 (56 kDa), TH-3 (58.1 kDa), and TH-4 (58.5 kDa). Only humans express all four isoforms. |
| General description: | Tyrosine hydroxylase (TH) is encoded by the gene mapped to human chromosome 11p15. TH is a tetramer of four identical subunits, which is characterized with a regulatory, catalytic, and tetramerization domains. The enzyme utilizes tyrosine, tetrahydrobiopterin (BH4) and O2 as cosubstrates, and Fe2+ as a cofactor. |
| Immunogen: | rat tyrosine hydroxylase (TH). |
| Specificity: | The antibody recognizes an epitope present in the N-terminal region (approx. aa 9-16) of both rodent and human tyrosine hydroxylase. Clone TH-2 reacts with the intact TH subunits. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |