Monoclonal Anti-ε-Tubulin antibody produced in mouse
SIGMA/T1323 - clone TUB-11, purified immunoglobulin, buffered aqueous solution
MDL Number: MFCD03792420
Product Type: Chemical
Western Blotting Whole extract of cultured Chicken fibroblasts cells were separated on SDS-PAGE and probed with Monoclonal Anti-ε-Tubulin antibody produced in Mouse, Clone: TUB-11 (Cat. No. T1323). The antibody was developed using 1:1,000 Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A2179). Lanes 1. 4 μg/mL antibody 2. 2 μg/mL antibody 3. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | TUB-11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen 55 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | frog, mouse, chicken, human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: suitable |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 2-4 μg/mL using whole extract of cultured chicken fibroblasts | |
| UniProt accession no. | Q9UJT0  |
| Application: | Monoclonal Anti-ε-Tubulin antibody produced in mouse may be used in: • immunoblotting • enzyme-linked immunosorbent assay (ELISA) • immunofluorescence |
| Biochem/physiol Actions: | Tubulin ε 1 (ε-Tubulin) plays a vital role in microtubule anchoring and centriole maturation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-ε-Tubulin (mouse IgG2a isotype) is derived from the TUB-11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with a synthetic peptide. |
| General description: | Tubulin epsilon 1 (ε-tubulin) is a member of tubulin superfamily. ε-tubulin is localized to the centrosome in cell-cycle-dependent manner. It is encoded by the gene mapped to human chromosome 6q21. |
| Immunogen: | synthetic peptide corresponding to amino acids 352-366 of human ε-tubulin, conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| Specificity: | Monoclonal Anti-ε-Tubulin recognizes human, mouse, frog, and chicken ε-tubulin (55 kDa). |
| Storage and Stability: | For continuous use, store at 2-8 °C for up to one month.For prolonged storage, freeze in working aliquots at−20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also notrecommended. If slight turbidity occurs upon prolongedstorage, clarify the solution by centrifugation beforeuse. Working dilutions should be discarded if not usedwithin 12 hours. |
| WGK Germany | - |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |