Sigma 7-9®
SIGMA/T1378 - ≥99% (titration), powder or crystals
Synonym: Trizma® base; 2-
CAS Number: 77-86-1
Empirical Formula (Hill Notation): C4H11NO3
Molecular Weight: 121.14
EC Number: 201-064-4
MDL Number: MFCD00004679
Linear Formula: NH2C(CH2OH)3
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T1378-100G
This image depicts SKU: T1378-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T1378-500G
This image depicts SKU: T1378-500G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T1378-1KG
This image depicts SKU: T1378-1KG
| absorption | ≤0.4 at 290 at 40% |
| application(s) | clinical research diagnostic assay manufacturing life science and biopharma sample preparation |
| assay | ≥99% (titration) |
| bp | 219-220 °C/10 mmHg (lit.) |
| color | white to off-white |
| description | aminopeptidase substrate |
| form | powder or crystals |
| impurities | ≤0.5% water (Karl Fischer) |
| InChI | 1S/C4H11NO3/c5-4(1-6,2-7) |
| InChI key | LENZDBCJOHFCAS-UHFFFAOYSA |
| mp | 167-172 °C (lit.) |
| pH | 10.5-12 |
| pKa (25 °C) | 8.1 |
| Quality Level | 200  |
| SMILES string | NC(CO)(CO)CO |
| solubility | water: 0.667 g/mL, clear, colorless to faintly yellow |
| storage condition | dry at room temperature |
| technique(s) | ELISA: suitable |
| flow cytometry: suitable | |
| immunohistochemistry: suitable | |
| useful pH range | 7-9 |
| Application: | Sigma 7-9® has been used: • as a buffer for sample preparation in a study involving Enzyme-linked immunosorbent assay (ELISA) • as a buffer for sample preparation in research involving immunohistochemistry and immunofluorescence |
| Features and Benefits: | • Suitable for Biological and Biochemical Research • Effective Buffering from pH 7-9 (25 °C) with a pKa of 8.1 (25 °C) |
| General description: | Sigma 7-9® also known as Trizma base or tris(hydroxymethyl)aminom Additionally, Tris aids in enhancing the permeability of cell membranes. This compound is indispensable in laboratories where it is utilized to prepare buffers compatible with biological fluids, serve as a standard pH solution, and facilitate procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells. |
| Legal Information: | Sigma 7-9 is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | For additional information on our range of Biochemicals , please complete this form . |
| Other Notes: | The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. |
| Packaging: | 1 kg in poly bottle |
| Packaging: | 100 kg in fiber drum |
| Packaging: | 100, 500 g in poly bottle |
| Packaging: | 5, 10, 25, 50 kg in poly drum |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Purity | ≥99% (titration) |
| bp | 219-220 °C/10 mmHg (lit.) |
| mp | 167-172 °C (lit.) |
| UNSPSC | 12352104 |