Trizma® base
SIGMA/T1503 - Primary Standard and Buffer, ≥99.9% (titration), crystalline
Synonym: 2-
CAS Number: 77-86-1
Empirical Formula (Hill Notation): C4H11NO3
Molecular Weight: 121.14
EC Number: 201-064-4
MDL Number: MFCD00004679
Linear Formula: NH2C(CH2OH)3
Product Type: Chemical
absorption | ≤0.05 at 290 nm at 40% |
application(s) | diagnostic assay manufacturing |
assay | ≥99.9% (titration) |
bp | 219-220 °C/10 mmHg (lit.) |
description | aminopeptidase substrate |
form | crystalline |
InChI | 1S/C4H11NO3/c5-4(1-6,2-7) |
InChI key | LENZDBCJOHFCAS-UHFFFAOYSA |
mp | 167-172 °C (lit.) |
pH | 10.5-12 |
pKa (25 °C) | 8.1 |
Quality Level | 300 ![]() |
SMILES string | NC(CO)(CO)CO |
solubility | ethylene glycol: soluble 79.1 mg/mL at 25 °C |
methanol: soluble 26 mg/mL at 25 °C | |
water: soluble (678 g/l at 20 °C) | |
useful pH range | 7-9 |
Application: | Trizma® base has been used: • as a component of H buffer (cell dissociation buffer) • for washing and saturation of wells in double sandwich ELISA immunoenzymatic technique • as an assay buffer for reconstitution of extracted and dried protein samples • to prepare Tris-HCl buffer that is used to stabilize proteins • as a buffer to extract carotenoid from tubers • as a component of sample buffer during protein extraction prior to western blotting • as a component of sample buffer for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) • to prepare simulated body fluid (SBF) for calcium phosphate (CaP) resorption assay • as a buffer for polydopamine (PDA) deposition on stainless steel (SS) substrate |
General description: | 40% (w/w) solution in water is clear and colorless. |
General description: | Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight. |
Legal Information: | Trizma is a registered trademark of Sigma-Aldrich Co. LLC |
Other Notes: | Easily compare specifications for Trizma products with the Trizma specification table ![]() |
Other Notes: | The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. |
Packaging: | 1 kg in poly bottle |
Packaging: | 25, 100, 250, 500 g in poly bottle |
Packaging: | 5, 10, 25, 50 kg in poly drum |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥99.9% (titration) |
bp | 219-220 °C/10 mmHg (lit.) |
mp | 167-172 °C (lit.) |
UNSPSC | 12352104 |