Transforming Growth Factor-β1 from human platelets
SIGMA/T1654 - TGF-β1, lyophilized powder, suitable for cell culture, 1 × 106 U/mg
Synonym: TGF-β1
MDL Number: MFCD00166089
Product Type: Chemical
assay | ≥97% (SDS-PAGE and N-terminal analysis) |
biological source | human |
form | lyophilized powder |
impurities | ≤1 EU/μgtested (LAL test) |
mol wt | protein 25 kDa |
packaging | pkg of 1 μg |
pkg of 10 μg | |
potency | 0.04-0.3 ng/mL ED50/EC50 |
quality | endotoxin tested |
Quality Level | 200 ![]() |
specific activity | 1 × 106 U/mg |
storage condition | avoid repeated freeze/thaw cycles |
storage temp. | −20°C |
technique(s) | cell culture | mammalian: suitable |
UniProt accession no. | P01137 ![]() ![]() |
Analysis Note: | The biological activity is measured by its ability to inhibit the IL-4-dependent proliferation of mouse HT-2 cells. |
Application: | Transforming Growth Factor-β1 from human platelets has been used • to study the induction of a-smooth muscle actin by transforming growth factor-1 (TGF-β1) in breast cancer cells. • to study the efficiency of TGF-β1 in promoting embryonic stem-like cell proliferation with or without feeders. • as control to analyse the isolated TGF-β1 HPLC using reverse-phase chromatography. • to induce profibrotic response in renal fibroblasts and further study functional role for Smad3 (mothers against decapentaplegic homolog 3) T388 phosphorylation. |
Biochem/physiol Actions: | TGF-β1 plays a key role in embryogenesis and tissue homeostasis. TGF-β1 is fundamentally involved in differentiation by mediating adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation and immune cell function. TGF-β1 is a multifunctional peptide capable of influencing cell proliferation, migration, death, tissue growth, and other functions in a wide range of cell types. TGF-β1 is associated with the developmental and metastasis of colorectal cancer. Downregulation of TGF-β1 might lead to many disease conditions, including tumor formation. |
Biochem/physiol Actions: | Transforming growth factor-β1 (TGF-β1) is produced by many cell types, but is reported to be most concentrated in mammalian platelets, where it is present at approximately four times the level of TGF-β2. |
General description: | The biologically active form of TGF-β1 (transforming growth factor-β1 human) exists as a disulphide-linked homodimer. In mammals, three isoforms of TGF-β has been identified : TGF-β1, TGF-β2 and TGF-β3. TGF-β1 encodes a 25 kDa peptide. TGF-β1 precursor polypeptide is characterized with hydrophobic signal sequence, pro-region and mature peptide. Cleavage of the precursor at tetrabasic cleavage site results in monomers of carboxy-terminal 112 amino acids. The TGF-β1 gene is mapped to human chromosome 19q13.2. |
Physical form: | Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile, 0.1% TFA, containing 50 ug of BSA per 1 ug cytokine |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥97% (SDS-PAGE and N-terminal analysis) |
activity | specific activity: 1 × 106 U/mg |
Storage Temp. | −20°C |
UNSPSC | 12352200 |