Anti-TDP-43 antibody produced in rabbit
SIGMA/T1705 - ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-ALS10; Anti-TARDBP; Anti-TARDP43; Anti-Tar DNA binding protein 43
Product Type: Chemical
Immunohistochemistry Nuclear and cytoplasmic localization of TDP-43 in mouse kidney. Fixed tissue was stained with 5 μg/mL Anti-TDP-43 (Cat. No. T1705) followed by Goat Anti-Rabbit IgG, HRP conjugate (×40 magnification).
Immunofluorescence Nuclear and cytoplasmic localization of TDP-43 in HepG2 cells. Fixed cells were stained with 5 μg/mL Anti-TDP-43 (Cat. No. T1705) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.
Immunoblotting Tissue and cells extract were separated on SDS-PAGE, blotted with 1 μg/mL Anti-TDP-43 (Cat. No. T1705), and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Rat spinal cord; 2. Mouse lung; 3. HEK-293T; 4. K562; 5. HepG2; 6. U2OS; 7. U2OS. Antibody + TDP-43 immunizing peptide (human, 181-198)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~43 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 5-10 μg/mL using rat, mouse or human kidney |
| indirect immunofluorescence: 5-10 μg/mL using human HepG2 cells | |
| western blot: 1.5-3.0 μg/mL using human U2OS cell lysates | |
| UniProt accession no. | Q13148  |
| Biochem/physiol Actions: | Heterogeneous nuclear ribonucleoproteins (hnRNPs) are involved in the generation and processing of RNA, including transcription, splicing, transport and stability. TDP-43 regulates transcription of human immunodeficiency virus (HIV). The protein is identified as a major pathological protein, in both frontotemporal lobe degeneration subtype (FTLD-U) and amyotrophic lateral sclerosis (ALS). Abnormal phosphorylation of TDP-43 at Ser409/410 has also been observed in FTLD-U and ALS, suggesting a toxic gain of function leading to apoptosis. |
| Disclaimer: | Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | TDP-43 (TAR DNA binding protein, TARDP) is encoded by the gene mapped to human chromosome 1p36.22. The encoded protein belongs to the family of heterogenous nuclear ribonucleoproteins (hnRNPs) that bind single stranded RNA. TDP-43 is a 414 amino acid nuclear protein and is widely expressed in a variety of tissues. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | Anti-TDP-43 specifically recognizes human, mouse, and rat TDP-43. |
| Storage and Stability: | Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| WGK Germany | 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |