Trypsin Agarose
SIGMA/T1763 - buffered aqueous suspension, from bovine pancreas trypsin
MDL Number: MFCD00082094
Product Type: Chemical
| biological source | bovine pancreas (trypsin) |
| concentration | ≥15 units/mL (packed gel) |
| extent of labeling | ≥15 units per mL packed gel |
| form | buffered aqueous suspension |
| matrix | cross-linked beaded agarose |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| Application: | A very active and very stable trypsin agarose derivative has been used to optimize the design of the synthesis of a model dipeptide, benzoylarginine leucinamide. Trypsin has also been used in a study to investigate protonation-state determination in proteins using high-resolution X-ray crystallography. |
| Application: | Trypsin Agarose has been used for enzymatic hydrolysis of prolamins and gliadin to generate peptides. |
| General description: | The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-su |
| General description: | Trypsin Agarose is an insoluble enzyme product. It is produced by reacting a conventional "soluble" enzyme (trypsin) with an inert base (agarose). This insoluble conjugate retains the activity of the original enzyme. Trypsin bound to agarose are highly stable and maintain denaturing conditions for longer time than the soluble trypsin. |
| Other Notes: | Insolubilized |
| Packaging: | 50 units in glass bottle |
| Physical form: | Suspension in approx. 10 mM acetic acid, pH 3.2 |
| Unit Definition: | One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 °C (titrimetric assay). |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H317 - H319 - H335 |
| Precautionary statements | P280 - P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42/43 |
| Safety Statements | 22-24-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.4.21.4 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |